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Phenol-degradation microbial agent, immobilized globules and preparation method

A technology of microbial agent and immobilized pellets, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of secondary pollution and high cost of phenol, achieve stable degradation environment, prolong the use time and Frequency, effects of adaptation to toxic environment

Inactive Publication Date: 2018-02-02
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the disadvantages of secondary pollution and high cost in the removal of phenol by physical and chemical methods, microbial removal of phenol in groundwater is one of the research hotspots.

Method used

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  • Phenol-degradation microbial agent, immobilized globules and preparation method
  • Phenol-degradation microbial agent, immobilized globules and preparation method
  • Phenol-degradation microbial agent, immobilized globules and preparation method

Examples

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preparation example Construction

[0030] The preparation method of phenol-degrading microbial bacterial agent of the present invention comprises the following steps:

[0031] (1) Select the surface filter material of the biological manganese sand filter of the waterworks, inoculate and activate the culture to prepare the first bacterial liquid for the surface filter material, wherein "biological manganese sand filter" is a relatively mature biological removal method in the current waterworks Manganese process facilities, its surface filter material is easy to obtain, especially for northern cities in my country, the main source of raw water for waterworks is groundwater with high manganese content, and the surface filter material of the biological manganese sand filter of the present invention is then preferably from water sources that are high in manganese Waterworks with high groundwater content, the groundwater source water only contains a relatively high concentration of manganese ions, in addition, there is ...

Embodiment 1

[0051] The invention provides a method for preparing a microbial agent applied to the degradation of phenol pollution in groundwater. The microbial agent is separated and extracted from the surface filter material of a biological manganese sand filter in a waterworks in Shenyang whose water source is high-manganese groundwater. , the extraction method is as follows:

[0052](1) Take 10 g of the surface filter material of the biological manganese sand filter and inoculate it into the LB medium added with 10 glass beads. The pH of the culture medium was adjusted to 7.25-7.3 before sterilization, and the sterilization condition was 121°C for 20 minutes. After sterilization, 2.38 g / L HEPES buffer solution with pH adjusted to 7.25-7.3 was added through a 0.22 μm sterile filter, 28 After activation at 150 rpm / min for 24 h at ℃, inoculate the activated bacterial liquid again into LB medium sterilized at 121 °C for 20 min, and incubate at 150 rpm / min at 28 °C for 12 h to prepare bacte...

Embodiment 2

[0063] The present embodiment provides a kind of microbial treatment method that is applied to moderate phenol pollution in groundwater, and it comprises the following steps:

[0064] Obtain the bacterium suspension of microbial bacterial agent according to embodiment 1.

[0065] Prepare phenol-containing carbon-source-free MSVP medium, the medium components are: (NH 4 ) 2 SO 4 0.24g / L, MgSO 4 ·7H 2 O0.2g / L, anhydrous CaCl 2 0.06g / L, KH 2 PO 4 0.02g / L, Na 2 HPO 4 12H 2 0.03g / L, adjust the pH to 7.25-7.3, sterilize at 121°C for 20min, add 2.38g / L HEPES, 0.002g / lm w 12 , 1mg / L, 5mg / L, 10mg / L phenol solution, prepared into a series of concentration gradient phenol wastewater.

[0066] Take a certain amount of bacterial suspension and add it to the above-mentioned phenol wastewater containing medium to degrade phenol. The method for determining the concentration of phenol and the method for determining the degradation rate are the same as in Example 1. Please refer t...

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Abstract

The invention provides a phenol-degradation microbial agent, immobilized globules and a preparation method. Microorganisms in the phenol-degradation microbial agent are separated and extracted from surface filter materials of a biological manganese sand filter pool of a waterworks, and after the microorganisms are cultured and screened in a manganese-contained MSVP liquid medium and a manganese-contained MSVP solid medium and are activated in a TBS medium, the phenol-degradation microbial agent is prepared. The invention further provides the preparation method for preparing the phenol-degradation microbial agent into the immobilized globules. According to the preparation method, the gel globules are prepared by using cross-linking polymerization products of silicon dioxide or manganese dioxide and sodium alginate as carriers and embedding the phenol-degradation microbial agent. The phenol-degradation microbial agent can be applied to rapidly degrading phenol at low-temperature oxygen-free and neutral conditions of underground water. The phenol-degradation microbial agent immobilized globules can provide stable degradation environment, are more suitable for toxic environment, and are used for treating phenol pollution of the underground water with higher concentration.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of organic pollutants in groundwater, and in particular relates to a phenol-degrading microbial agent and a preparation method and application thereof. Background technique [0002] Phenol is a common organic pollutant and the most toxic phenolic compound. It is difficult to degrade in the natural state, and the residue can remain in the environment for a long time, polluting the environment for a long time. Phenol is an important raw material for organic synthesis. It is widely used in papermaking, pesticides, textiles, dyes, medicines, plastics and other industries, as well as in the production of surfactants, explosives, fertilizers, paint removers, antioxidants, and curing agents. Phenol is extremely harmful to the human body and the environment. Contact with the human body can cause skin burns, damage to nerves, liver and kidneys, etc. Long-term drinking of phenol-contaminated ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14C12N11/10C02F3/34C12R1/38C12R1/01C12R1/15C12R1/20C02F103/06C02F101/34
CPCC02F3/34C02F2101/345C02F2103/06C12N1/20C12N11/10C12N11/14
Inventor 席北斗姜永海杨昱廉新颖孙晓玲马志飞彭星徐祥健
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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