A chitosan-based gradient biomimetic composite scaffold material and its construction method

A composite scaffold and construction method technology, applied in medical science, prosthesis, etc., to achieve stable structure, uniform pore size, and good connection effect

Active Publication Date: 2019-06-21
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant reports on the composite of chitosan, β-sodium glycerophosphate, gelatin, and nano-hydroxyapatite as scaffold materials for biomimetic gradient repair of cartilage tissue engineering.

Method used

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  • A chitosan-based gradient biomimetic composite scaffold material and its construction method
  • A chitosan-based gradient biomimetic composite scaffold material and its construction method
  • A chitosan-based gradient biomimetic composite scaffold material and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation of Cs / GP / Gel (mass ratio 1:15.2:1.8) physical blending composite scaffold material (as a comparative example)

[0039] Weigh 2.2 g of chitosan powder, add it into 100 mL of 0.1 mol / L acetic acid solution, and stir magnetically for 2 h at 40° C. to make it evenly mixed. Centrifuge at 1000rpm for 0.5h to degas the chitosan solution and remove slag. Weigh 20.0 g of sodium β-glycerophosphate powder, add it into 20 mL of deionized water, and stir magnetically at room temperature for 30 min to dissolve it evenly. Weigh 6.0 g of gelatin particles, add to 100 mL of deionized water, and stir magnetically for 30 min at 40°C to make them evenly mixed. At room temperature, the prepared chitosan solution was stirred rapidly on a magnetic stirrer, and the β-sodium glycerophosphate solution was slowly added dropwise during the stirring process, and the solution was fully stirred for 30 minutes to prepare a Cs / GP solution. Observe that there is no obvious floccu...

Embodiment 2

[0040] Example 2 Preparation of Cs / GP / Gel (mass ratio 1:6.7:3.1) physical blending composite scaffold material (as a comparative example)

[0041] Weigh 2.5 g of chitosan powder, add it into 100 mL of 0.1 mol / L acetic acid solution, and stir magnetically for 3 h at 50° C. to make it evenly mixed. Centrifuge at 1000rpm for 0.5h to degas the chitosan solution and remove slag. Weigh 24.0 g of sodium β-glycerophosphate powder, add it to 16 mL of deionized water, and stir magnetically at room temperature for 40 min to dissolve it evenly. Weigh 7.0 g of gelatin particles, add to 100 mL of deionized water, and stir magnetically for 40 min at 50°C to make them evenly mixed. At room temperature, the prepared chitosan solution was stirred rapidly on a magnetic stirrer, and the β-sodium glycerophosphate solution was slowly added dropwise during the stirring process, and the solution was fully stirred for 30 minutes to prepare a Cs / GP solution. Observe that there is no obvious flocculen...

Embodiment 3

[0042] Example 3 Preparation of Cs / GP / Gel (mass ratio 1:8.4:1.5) physical blending composite scaffold material (as a comparative example)

[0043]Weigh 3.0 g of chitosan powder, add it into 100 mL of 0.1 mol / L acetic acid solution, and stir magnetically for 4 h at 50° C. to make it evenly mixed. Centrifuge at 1000rpm for 1h to degas and deslag the chitosan solution. Weigh 30.0 g of sodium β-glycerophosphate powder, add it to 10 mL of deionized water, and stir magnetically at room temperature for 60 min to dissolve it evenly. Weigh 8.0 g of gelatin particles, add to 100 mL of deionized water, and mix evenly with a magnetic stirrer at 60°C for 60 min. At room temperature, the prepared chitosan solution was stirred rapidly on a magnetic stirrer, and the β-sodium glycerophosphate solution was slowly added dropwise during the stirring process, and the solution was fully stirred for 30 minutes to prepare a Cs / GP solution. Observe that there is no obvious flocculent substance in th...

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Abstract

The invention discloses a chitosan-based gradient bionic composite stent material and a construction method thereof, and belongs to the field of tissue engineering materials. Isopyknic Cs / GP / Gel solution and acetic acid solution are added into a vessel A and a vessel B respectively which are horizontally communicated through a communicating pipe, the Cs / GP / Gel solution continuously flows into a stent mold, and the acetic acid solution continuously flows into the vessel B; when the solutions in the two vessels continuously flow into the stent mold, a stent pre-product is taken out after pre-frozen treatment, and a Cs / GP / Gel gradient composite stent is obtained by preparation; during the preparation of the Cs / GP / Gel gradient composite stent, when residual liquor in the vessel B is one fourthto one fifth, a Cs / GP / Gel / nHAp solution is added into the vessel B, and other steps are the same as those of the Cs / GP / Gel gradient composite stent. The aperture of the gradient composite stent prepared with the method shows obvious gradient progressivity, and pores are mutually communicated, so that cartilage calcification layer and cartilage layer simulated by nHAp have a good connection effect, and are applicable to the field of cartilage tissue engineering.

Description

technical field [0001] The invention belongs to the field of tissue engineering materials, and particularly relates to a method for constructing tissue engineering gradient bionic cartilage scaffolds by compounding chitosan, sodium beta-glycerophosphate, gelatin and nano-hydroxyapatite. Background technique [0002] The osteochondral tissue structure of normal joints is mainly composed of three parts: hyaline cartilage, interface structure (calcified cartilage layer) and subchondral bone, showing obvious gradient and complex physiological characteristics in structure. Due to the huge amount of articular cartilage used in various activities, it is very easy to cause damage in trauma and acute and chronic inflammation, and due to the suddenness and complexity of stress, cartilage wear, tear, fracture and comminuted damage are mostly present Wait. Joint prosthesis replacement is one of the effective treatment methods at present, but surgical joint replacement is not only expen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/12A61L27/00A61L27/58A61L27/50A61L27/56
Inventor 宋克东卢延国李丽颖李文芳刘天庆
Owner DALIAN UNIV OF TECH
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