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Maize salt stress response miRNA MiR164 and application thereof

A salt stress, maize technology, applied in maize salt stress response miRNAMiR164 and its application field, can solve problems such as the decline of rice salt tolerance

Inactive Publication Date: 2018-01-23
NANTONG UNIVERSITY
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Overexpression of osa-MIR396c significantly reduces the salt tolerance of rice

Method used

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  • Maize salt stress response miRNA MiR164 and application thereof
  • Maize salt stress response miRNA MiR164 and application thereof
  • Maize salt stress response miRNA MiR164 and application thereof

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Experimental program
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Embodiment 1

[0014] 1. Salt stress maize seedlings

[0015] Choose 200mM, 250mM NaCl solution to treat corn seedlings, and use 0mM NaCl solution as a control. Before studying the salt stress response miRNA in corn, it is necessary to determine the appropriate salt stress treatment material and concentration, and under different degrees of salt stress, obtaining phenotypic data with significant differences is the basis for accurate identification of corn salt stress response miRNA. crucial step. After a lot of experiments, it was finally determined that the NaCl solution above 200mM has research value. Therefore, under the treatment of three concentrations of NaCl solution, according to the phenotype identification results of the corn inbred line LH196 seedlings and related data statistics, it was finally determined that the salt stress concentration for phenotype identification was set as 200mM and 250mM, and the 250mM NaCl solution treatment Maize seedlings were used as experimental mat...

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Abstract

The invention relates to the field of plant stress resistance and in particular to maize salt stress response miRNA MiR164 and application thereof. The nucleotide sequence of the gene is as shown in SEQ ID No.1. According to the maize salt stress response miRNA, under salt stress, expression of zma-miR164 is reduced, target genes GRMZM2G114850 (NAC transcription factor family proteins, NAM, ATAF and CUC) and GRMZM2G008819 both have remarkable expression increase, and the NAC transcription factor responds to salt marsh stress by regulating and controlling expression modes of downstream genes.

Description

technical field [0001] The invention relates to the field of plant stress resistance, in particular to corn salt stress response miRNA MiR164 and application thereof. Background technique [0002] miRNA functions are diverse, and salt stress, drought stress, and ABA can all induce the up-regulated expression of miR417. Interestingly, the seed germination rate of Arabidopsis overexpressing miR417 will be reduced under salt stress. Under salinity and drought stress, two members of the Arabidopsis miR395 family, miR395c and miR395e, the former negatively regulates Arabidopsis seed germination, while the latter positively regulates seed germination. The same miRNA has different regulatory modes on plants under different stresses. For example, overexpression of miR402, under salt stress, not only accelerates Arabidopsis seed germination but also accelerates seedling growth. However, under low temperature or drought stress, only Can promote seed germination, can not promote the g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00A01H6/46
Inventor 汪保华李平付蓉谢永宏赵银川何雪川
Owner NANTONG UNIVERSITY
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