Pesticide residue rapid detection method based on nanogold model enzyme
A technology for pesticide residues and detection methods, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problem of harsh reaction conditions, high cost, and difficulty in natural enzyme modification. Preservation and other issues, to achieve the effect of low environmental conditions, low cost, and good stability
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Embodiment 1
[0039] 1. Preparation method of AuNPs:
[0040] (1) Preparation method of seed solution: Add 5 mL of HAuCl with a concentration of 0.5 mM to 5 mL of CTAB solution with a concentration of 0.2 mol / L in a constant temperature water bath at 30 °C 4 Aqueous solution, after stirring and mixing, add 0.6mL sodium borohydride solution with a concentration of 0.01M and place it in an ice-water bath. A gold seed sol containing gold nanocrystals.
[0041] (2) Preparation of AuNPs: 150 μL of 4 mM silver nitrate solution and 5 mL of 0.5 mM HAuCl were added to 5 mL of 0.2 M CTAB solution 4 Solution, after stirring evenly, slowly add 70 μL of vitamin C with a concentration of 78.8 mM, vitamin C will immediately reduce the solution from brown to colorless, and stir the solution at 700 r / min for 2 minutes to obtain a growth solution; add 12 μL of gold crystal seed sol Stir in 10mL of growth solution at 200r / min for 3min, the solution turns from colorless to purple blue, place the solution at ...
Embodiment 2
[0057] 1. The preparation method of AuNPs: with specific embodiment 1.
[0058] 2. Establish a standard curve for organophosphorus pesticides (OPs) dichlorvos:
[0059] (1) Dichlorvos standard solution preparation: Dilute the dichlorvos standard with distilled water to a series of concentrations of 0 μL / mL, 20 μL / mL, 40 μL / mL, 60 μL / mL, 80 μL / mL, and 100 μL / mL;
[0060] (2) Preparation of chromogenic AB solution: preparation of solution A: take 0.012g TMB and dilute it with absolute ethanol to a 10mL volumetric flask, and ultrasonically oscillate for 3 minutes to obtain a TMB solution with a concentration of 1mM; preparation of solution B: take 0.575gH 2 o 2 Add it into a 10mL volumetric flask, make it up to volume with distilled water, shake evenly to get a concentration of 10mM H 2 o 2 Solution; AB solution needs to react within 2 hours after preparation.
[0061] (3) To establish a standard curve: take 40 μL of dichlorvos with a concentration of 0 μL / mL, 20 μL / mL, 40 μL...
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