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Preparation method and application of a bacterial strain for preventing and treating potato wilt and its preparation

A fusarium wilt and potato technology, applied in the field of preparation of bacterial strains and their preparations, can solve the problems that Bacillus subtilis potato wilt has not been found, cannot be completely cured, and endangers ecological balance, etc., to inhibit the growth of pathogenic bacteria, protect the environment, and promote reproduction Effect

Active Publication Date: 2018-06-01
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional methods, such as crop rotation with gramineous crops or green manure crops, application of organic fertilizers, and strengthening of water and fertilizer management, etc., can reduce the disease, but cannot be completely cured, and the applicability is poor
The application of metalaxyl, carbendazim and other medicaments has a certain effect in a short period of time, but the effect is not long-lasting, and it pollutes the environment and endangers the ecological balance
[0005] The use of microorganisms to control plant soil-borne diseases is one of the most effective control measures. After searching, there is no report on the use of Bacillus subtilis (B.subtilis) to control potato wilt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A bacterial strain for preventing and treating potato wilt. The bacterial strain is Bacillus subtilis MES810, which is preserved in the China Common Microorganism Culture Collection and Management Center. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date As of August 10, 2017, the deposit number is CGMCCNo.14514. The strain of Bacillus subtilis MES810 was isolated from the potato field by our company's R&D personnel. After streaking on a plate, it was cultured at 32°C for 24 hours to make a slant strain and refrigerated at 4°C. Then a single colony was picked, sectioned, stained with crystal violet, and examined under a microscope to determine that it was Bacillus subtilis. Taking the isolated potato fusarium wilt fungus Fusarium as the target, a plate confrontation experiment was carried out on it with Bacillus, cultured at 30°C, and after 4 days, it was found that the strain of Bacillus could significant...

Embodiment 2

[0038] A bacterial strain for preventing and treating potato wilt. The bacterial strain is Bacillus subtilis MES810, which is preserved in the China Common Microorganism Culture Collection and Management Center. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date As of August 10, 2017, the deposit number is CGMCCNo.14514. The strain of Bacillus subtilis MES810 was isolated from the potato field by our company's R&D personnel. After streaking on a plate, it was cultured at 32°C for 24 hours to make a slant strain and refrigerated at 4°C. Then a single colony was picked, sectioned, stained with crystal violet, and examined under a microscope to determine that it was Bacillus subtilis. Taking the isolated potato fusarium wilt fungus Fusarium as the target, a plate confrontation experiment was carried out on it with Bacillus, cultured at 30°C, and after 4 days, it was found that the strain of Bacillus could significant...

Embodiment 3

[0049] A bacterial strain for preventing and treating potato wilt. The bacterial strain is Bacillus subtilis MES810, which is preserved in the China Common Microorganism Culture Collection and Management Center. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date As of August 10, 2017, the deposit number is CGMCCNo.14514. The strain of Bacillus subtilis MES810 was isolated from the potato field by our company's R&D personnel. After streaking on a plate, it was cultured at 32°C for 24 hours to make a slant strain and refrigerated at 4°C. Then a single colony was picked, sectioned, stained with crystal violet, and examined under a microscope to determine that it was Bacillus subtilis. Taking the isolated potato fusarium wilt fungus Fusarium as the target, a plate confrontation experiment was carried out on it with Bacillus, cultured at 30°C, and after 4 days, it was found that the strain of Bacillus could significant...

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Abstract

The invention relates to a preparation method and application of a bacterial strain for preventing and treating potato wilt and its preparation. The bacterial strain is Bacillus subtilis MES810, the preservation date is August 10, 2017, and the preservation number is CGMCCNo.14514. The preparation method of the preparation corresponding to the bacterial strain is as follows: activation of the strain; preparation of the primary seed liquid; preparation of the secondary seed liquid: fermenting in a fermenter, and discharging to obtain a liquid preparation of Bacillus subtilis. The Bacillus subtilis of the present invention has a strong inhibitory effect on the Fusarium pathogenic bacteria composed of Fusarium solani, Fusarium oxysporum, Fusarium moniliforme, Fusarium nivale or Fusarium elderberry and Fusarium avenum, preventing potato Fusarium wilt occurs, and the control effect reaches more than 80%, which promotes the increase of potato production and income. The microbial bacterial agent of the present invention has the characteristics of no phytotoxicity, no residue, no inhibition of growth, less dosage and simple application method, which provides possibility for diversification of application schemes and protection of ecological environment.

Description

technical field [0001] The invention belongs to the field of agricultural biology, and relates to a preparation method of a bacterial strain for preventing and treating potato wilt and a preparation thereof. Background technique [0002] The invention relates to a bacterial strain for preventing and treating potato wilt and a preparation method thereof, and belongs to the technical field of using microorganisms to prevent and control plant soil-borne diseases. [0003] Potato Fusarium wilt is a devastating soil-borne fungal disease. Once it occurs on a large scale, it is difficult to cure it, which can lead to a 20%-30% reduction in potato production, and up to 50%-80% in severe cases, or even extinction. my country is the country with the largest potato planting area in the world, and potato is one of the four major food crops after rice, wheat, and corn. Potato wilt is an important fungal soil-borne disease that has caused damage to potato in recent years. When the disease...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
Inventor 唐广辉赵钢勇顾欣燕孙海远闫辉敏
Owner 天津开发区坤禾生物技术有限公司
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