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A method for extracting chondroitin sulfate from fish bones

A chondroitin sulfate and fish bone technology, applied in the field of chondroitin sulfate extraction, can solve problems such as complicated steps, destruction of chondroitin sulfate, low product yield, etc., and achieve the effects of reasonable method steps, wide application prospect, and low allergy

Active Publication Date: 2020-08-04
SHANDONG HOMEY AQUATIC DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most used method is to extract chondroitin sulfate by combining dilute alkali or concentrated alkali with enzymatic hydrolysis. This alkaline enzymatic hydrolysis production process has cumbersome steps and a long cycle. In the process of decolorization and decolorization, chondroitin sulfate is severely damaged, the product yield is low, the consumption of ethanol is large, and the production cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] In the present embodiment, a kind of method for extracting chondroitin sulfate from fish bone, it goes through the following steps:

[0026] (1) Select fresh marine fish bones as raw materials, cook for 35 minutes, and use high-pressure air to separate to obtain fish meat protein and fish bones;

[0027] (2) Washing and crushing the fish bones obtained in step 1 to an average particle size of 0.3 mm to obtain fish bone powder;

[0028] (3) Add the fishbone powder obtained in step 2 to 2.5 times the weight of the enzymolysis solution containing 2400 U / mL trypsin and 4800 U / mL papain, and adjust the pH to 9.0; then Enzymolysis for 20 hours, heated to 80 degrees Celsius, filtered to obtain clear liquid;

[0029] (4) Add ethanol to the clear liquid obtained in step 3 so that the mass concentration of ethanol reaches 70%, and collect the precipitate;

[0030] (5) The precipitate obtained in step 4 is slowly rinsed and washed with a detergent 1.2 times its weight, and the d...

Embodiment 2

[0033] In the present embodiment, a kind of method for extracting chondroitin sulfate from fish bone, it goes through the following steps:

[0034] (1) Select fresh marine fish bones as raw materials, cook for 20 minutes, and use high-pressure air to separate to obtain fish meat protein and fish bones;

[0035] (2) Washing and crushing the fish bones obtained in step 1 to an average particle size of 0.5 mm to obtain fish bone powder;

[0036] (3) Add the fishbone powder obtained in step 2 to 2 times the weight of the enzymatic hydrolysis solution containing 4000U / mL trypsin and 6000U / mL papain, and adjust the pH to 9.5; then enzymatic Decompose for 24 hours, heat to 85 degrees Celsius, and filter to obtain clear liquid;

[0037] (4) Add ethanol to the clear liquid obtained in step 3 so that the mass concentration of ethanol reaches 70%, and collect the precipitate;

[0038] (5) The precipitate obtained in step 4 is rinsed and washed with a detergent 1.5 times its weight, and...

Embodiment 3

[0041] In the present embodiment, a kind of method for extracting chondroitin sulfate from fish bone, it goes through the following steps:

[0042] (1) Select fresh marine fish bones as raw materials, cook for 40 minutes, and use high-pressure air to separate to obtain fish meat protein and fish bones;

[0043] (2) Washing and crushing the fish bones obtained in step 1 to an average particle size of 0.1 mm to obtain fish bone powder;

[0044] (3) Add the fishbone powder obtained in step 2 to a 4-fold weight enzymolysis solution containing 2000U / mL trypsin and 4000U / mL papain, and adjust the pH to 8.5; then enzymatically Decompose for 12 hours, heat to 75 degrees Celsius, and filter to obtain clear liquid;

[0045] (4) Add ethanol to the clear liquid obtained in step 3 so that the mass concentration of ethanol reaches 70%, and collect the precipitate;

[0046] (5) The precipitate obtained in step 4 is rinsed and washed with a detergent 0.5 times its weight, and the detergent ...

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Abstract

The invention discloses a method for extracting chondroitin sulfate for fish bones. The method comprises the following steps: (1) fresh sea fish bones are used as raw materials, steaming and boiling are carried out, high pressure air is used for separation, in order to obtain fish flesh protein and fish bones; (2) fish bones obtained in the step (1) are cleaned and crushed, in order to obtain thefish bone powder; (3) fish bone powder obtained in the step (2) is added into enzymatic hydrolysate, heating and filtering are carried out, in order to obtain clear liquid; (4) ethanol is added into the clear liquid obtained in the step (3), and precipitates are collected; (5) precipitates obtained in the step (4) are leached and washed with a washing agent; (6) water is added into the precipitates obtained in the step (5) till dissolving, ethanol is added, and precipitates are collected and dried. Enzymatic hydrolysis and a washing method are combined in order to obtain chondroitin sulfate with lower anaphylaxis and other excellent characteristics. The method has reasonable steps, advanced technology, and feasible operation, and is suitable for extraction of chondroitin sulfate.

Description

technical field [0001] The invention relates to the preparation of chondroitin sulfate, in particular to a method for extracting chondroitin sulfate from fish bones. Background technique [0002] Chondroitin sulfate is a type of glycosaminoglycan covalently linked to proteins to form proteoglycans. It is widely distributed in the extracellular matrix and cell surface of animal tissues. It is mainly used as a drug for treating rheumatism and rheumatoid diseases. It is also used as a functional As the main raw material of food, it also has anti-cancer, anti-thrombotic, and blood-lipid-lowering effects. It is now exported to the United States and many European countries as a raw material drug, and the market demand is strong. Most domestic production enterprises use cartilage as raw material to prepare chondroitin sulfate. There are three main production methods: salt hydrolysis, alkali hydrolysis and enzymatic hydrolysis. The most used method is to extract chondroitin sulfat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
Inventor 孙永军鞠文明胡炜唐晓波
Owner SHANDONG HOMEY AQUATIC DEV
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