Ruppia sinensis seeds storage method
A technology for the preservation of the grass and seeds of the grass, which is applied in the field of seed preservation of the grass, which can solve the problems that the population of the grass cannot be increased or even decreased, the seedlings cannot resist the dry exposure and the cold in winter, and the lack of a long-term storage method for the seeds of the grass, etc., to achieve Restoring a good ecological environment, promoting the maintenance of dormancy, and prolonging the effect of storage time
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[0031] Use distilled water and seawater to prepare 2000 milliliters of artificial seawater with a salinity of 10, 20, 30, 40, 50 and 60 respectively. The prepared artificial seawater was sterilized and placed in 12 1000ml wide-mouth glass bottles respectively. Randomly select 12,000 R. sinensis seeds, put them equally into jars filled with the above-mentioned sterilized seawater with different salinities, seal them, and place them at 0 or 4°C respectively. The seeds can be stored for 9 months. During the period, the sterilized artificial seawater with equal salinity and corresponding temperature was replaced once a month (that is, the artificial seawater placed at 0°C was still at 0°C), and the status of the seeds was checked every 3 months, and the germinated seeds were counted and recorded. Randomly select the ungerminated seeds of G. siberiani for seed viability test (germination experiment: 150 seeds are randomly taken out from each preservation experiment group, divided ...
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