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Application of polysaccharide of flowers of Apios americana Medikus to reduction of lipidosis in hepatocytes

A lipid deposition and hepatocyte technology, applied in the field of medicine, can solve the problems of easy rebound and lower body immunity, and achieve the effect of expanding the application field, reducing the lipid deposition of HepG2 cells, and having a good development prospect.

Active Publication Date: 2018-01-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the other weight loss and fat reduction methods also have the disadvantages of lowering the body's immunity and easy rebound.

Method used

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  • Application of polysaccharide of flowers of Apios americana Medikus to reduction of lipidosis in hepatocytes
  • Application of polysaccharide of flowers of Apios americana Medikus to reduction of lipidosis in hepatocytes
  • Application of polysaccharide of flowers of Apios americana Medikus to reduction of lipidosis in hepatocytes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1, a kind of preparation method of taro flower polysaccharide, carries out following steps successively:

[0067] 1), preparation of taro flower polysaccharide crude product:

[0068] According to the solid-liquid ratio of 1g:5ml, weigh 100g of taro flower and add 500mL of distilled water, extract in 90°C water bath for 80min (enzyme inactivation is also achieved during extraction), then beat, and then centrifuge (4000r / min for 15min), and Suction filter the supernatant obtained by centrifugation and collect the filtrate;

[0069] Use the filter residue obtained by centrifugation to replace the taro flower, repeat the above process of adding distilled water, then extracting (90°C water bath for 2 hours), beating, and centrifuging twice, and combine the filtrate obtained by centrifuging and suctioning for three times to concentrate to the original volume. 1 / 5 of the concentrated solution.

[0070] Under the condition of stirring, add ethanol (pure ethanol) t...

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Abstract

The invention discloses application of the polysaccharide of the flowers of Apios americana Medikus to reduction of lipidosis in hepatocytes. A preparation method for the polysaccharide of the flowersof Apios americana Medikus comprises the following steps: 1) preparation of a crude polysaccharide product of the flowers of Apios americana Medikus: mixing the flowers of Apios americana Medikus with distilled water, carrying out extraction at first, then carrying out smashing, centrifugation and concentration, adding ethanol in the obtained concentrate until the final concentration of ethanol is 80 + / - 5%, carrying out standing at 4 + / - 1 DEG C for 24 + / - 2 h, and then collecting a precipitate so as to obtain the crude polysaccharide product of the flowers of Apios americana Medikus; and 2)purification of the crude polysaccharide product of the flowers of Apios americana Medikus: subjecting the crude polysaccharide product of the flowers of Apios americana Medikus to decoloring with active carbon and Sevage-process deproteinization.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of taro flower polysaccharide in reducing lipid deposition in liver cells. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, Jinhua, Longyou, Wenzhou and other places. Studies have shown that different parts of taro (flowers, vines, leaves, tubers) are rich in free amino acids, soluble proteins, polysaccharides, saponins, flavonoids, isoflavones, vitamin C, vitamin E and minerals. Among them, taro f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P1/16A23L33/125C08B37/00
Inventor 郑晓冬楚强陈鹏华
Owner ZHEJIANG UNIV
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