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Indoor breeding method for adoxophyes braconid

A technology of the brown scalloped wasps and parasitic wasps, which can be applied in the fields of application, animal feed, animal feed, etc., and can solve problems such as obstacles to the use of the insects

Active Publication Date: 2018-01-16
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few domestic and foreign reports on the research on the indoor breeding technology of the brown banded moth, which has caused obstacles to the use of the pest in biological control. Feeding method becomes extremely urgent

Method used

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  • Indoor breeding method for adoxophyes braconid
  • Indoor breeding method for adoxophyes braconid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Place 1 senior larvae of S. mellonella in a ventilated 2ml centrifuge tube, and insert 2 newly emerged female adults and 1 male adult of Braconidus moth. Placed in a 25°C incubator. Observe the oviposition of parasitic wasps. After the parasitic wasps are covered with parasitic wasp eggs, the parasitic wasps are taken out and re-inoculated into a centrifuge tube containing an advanced larva of the wax moth. Repeat this step until the female parasitic wasps The bees died, repeated three times, and counted the number of eggs laid. The results are shown in Table 1. The host whose body surface was covered with parasitoid eggs was collected, placed in an incubator for further cultivation, the parasitoid eggs hatched into larvae, the number of larvae was counted, and the hatching rate was calculated. The results are shown in Table 1. After the larvae make cocoons and pupate, collect the bee pupae and store them in an incubator at 10°C.

[0017] The preserved bee pupae were ...

Embodiment 2

[0019] Put 1 prepupa of Orythia spp. into a ventilated 2ml centrifuge tube, and insert 2 newly emerged female adults and 1 male adult of the newly emerged Twister moth. Placed in a 25°C incubator. Observe the oviposition of the parasitic wasps. After the body of the red-veined ear borer is covered with the eggs of the parasitic wasps, take out the parasitic wasps and re-inoculate them in a centrifuge tube with a prepupa of the wax moth moth. Repeat this step until the parasitic wasps The female bees died, repeated three times, and counted the number of eggs laid. The results are shown in Table 1. The host whose body surface was covered with parasitoid eggs was collected, placed in an incubator for further cultivation, the parasitoid eggs hatched into larvae, the number of larvae was counted, and the hatching rate was calculated. The results are shown in Table 1. After the larvae make cocoons and pupate, collect the bee pupae and store them in an incubator at 12°C.

[0020] T...

Embodiment 3

[0022] Place 30 ear borers in a 15 × 10 × 5 cm insect-raising box, the top of which is open and covered with 200-mesh nylon gauze. Inoculate 60 female adults and 30 male adults of Braconis spondyloides into the insect rearing boxes. Placed in a 25°C incubator. Observe the oviposition situation of the parasitic wasps, when most (not less than 70% of the total insect population) of the parasitic wasps are all over the parasitic wasp eggs, the parasitic wasps are taken out and re-inoculated in the insect culture box containing 30 red veined ear borers After that, repeat this step until the parasitoid female wasps die, repeat three times, count the number of eggs laid, and the results are shown in Table 1. The host whose body surface was covered with parasitoid eggs was collected, placed in an incubator for further cultivation, the parasitoid eggs hatched into larvae, the number of larvae was counted, and the hatching rate was calculated. The results are shown in Table 1. After ...

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Abstract

The invention belongs to the field of biological control of agricultural and forest pests and relates to an indoor breeding method for adoxophyes braconid. The method comprises the following steps: putting a host into feeding ware, inoculating adult adoxophyes braconid, culturing in an incubator, enabling the body surface of the host to be full of parasitic braconid eggs, and replacing a new hostto continue inoculating until all female parasitic braconids die; hatching the parasitic braconid eggs into larva, enabling the larva to spin cocoons and pupate, and respectively collecting honey beepupae and new feathered adults to perform low temperature preservation so as to be used during field pest area release. According to the habit that the adoxophyes braconid can be parasitic, tirathabarufivena walker and galleria mellonella are selected as the host to perform domestic reproduction, large-scale production is realized, a pest source is provided for large-scale application of the adoxophyes braconid in production, the adoxophyes braconid can be preserved at a low temperature after the parasitic braconid is pupated and feathered and can be released at the fixed point and fixed areain the field pest area, and a technical support is provided for biological control of palm plants.

Description

technical field [0001] The invention belongs to the field of biological control of agricultural and forestry pests, and relates to a method for raising insects, in particular to an indoor breeding method for the brown-banded moth wasp. Background technique [0002] The red-veined ear borer is an important pest of palm plants, mainly harming areca nuts and coconuts. The larvae bore into the palm tidbits and heart leaf tissue, causing massive yield losses. Because palm plants are relatively tall, chemical control is difficult to implement and the effect is poor. At the same time, chemical control pollutes the environment and easily leads to insecticide resistance. According to the theory of biological control, using natural enemies to control pests is an effective way. [0003] Bracon adoxophyesi Mimankawa, also known as Bracon adoxophyesi Mimankawa, belongs to the genus Bracon adoxophyesi of the order Hymenoptera. The results of the investigation on the biological control r...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K50/90A23K10/20
Inventor 吕朝军钟宝珠覃伟权阎伟孙晓东刘博刘丽王晔楠李朝绪黄山春
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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