A method for preparing rice protein with highly soluble co-frame structure
A rice protein and soluble technology, which is applied in the field of food protein finishing, can solve problems such as food safety, nutrient loss, and destruction of peptide chain structures, and achieve improved solubility, emulsification, and amino acid balance characteristics, and broad application prospects Effect
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Embodiment 1
[0031] (1) Alkali dispersion: disperse 1000g of rice protein and 10g of casein in 10L of water, stir and mix well, and adjust the pH of the above protein dispersion to 10.0 with NaOH solution;
[0032] (2) Structure co-frame: Stir the feed solution obtained in step (1) for 60 min at a speed of 600r / min, so that the binding sites of the two proteins are fully exposed and the structure co-frame occurs;
[0033] (3) Acid neutralization: adjust the pH of the feed liquid obtained in step (2) to 7.0 with HCl;
[0034] (4) centrifugation: centrifuge the feed liquid obtained in step (3) at 4000g for 5min, and get the supernatant;
[0035] (5) Drying: Freeze-dry the supernatant obtained in step (4) to obtain a highly soluble structurally co-framed protein product.
Embodiment 2
[0037] (1) Alkali dispersion: disperse 1000g of rice protein and 1000g of casein in 10L of water, stir and mix well, and adjust the pH of the above protein dispersion to 11.0 with NaOH solution;
[0038] (2) Structure co-frame: Stir the feed solution obtained in step (1) for 90 minutes at a speed of 1500r / min, so that the binding sites of the two proteins are fully exposed and the structure co-frame occurs;
[0039] (3) Acid neutralization: adjust the pH of the feed liquid obtained in step (2) to 7.0 with HCl;
[0040] (4) centrifugation: the feed liquid obtained in step (3) is centrifuged at 7000g for 10min, and the supernatant is taken;
[0041] (5) Drying: Freeze-dry the supernatant obtained in step (4) to obtain a highly soluble structurally co-framed protein product.
Embodiment 3
[0043] (1) Alkali dispersion: disperse 1000g of rice protein and 2000g of casein in 10L of water, stir and mix well, and adjust the pH of the above protein dispersion to 12.0 with NaOH solution;
[0044] (2) Structure co-frame: Stir the feed solution obtained in step (1) for 30 minutes at a speed of 2000r / min, so that the binding sites of the two proteins are fully exposed and the structure co-frame occurs;
[0045] (3) Acid neutralization: adjust the pH of the feed liquid obtained in step (2) to 7.0 with HCl;
[0046] (4) centrifugation: centrifuge the feed liquid obtained in step (3) at 10000g for 20min, and get the supernatant;
[0047] (5) Drying: Freeze-dry the supernatant obtained in step (4) to obtain a highly soluble structurally co-framed protein product.
[0048] The properties of the finished products prepared in Examples 1-3 are shown in Table 1.
[0049] Table 1
[0050]
[0051] From the above analysis data, it can be concluded that the structure co-frame t...
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