Beta-amyloid plaque recognition and measurement method based on image processing

Abstract

The invention provides a beta-amyloid plaque recognition and measurement method based on image processing, and belongs to the technical field of biological image processing. The method comprises the following concrete steps that a model organism brain slice is subjected to immunostaining by a beta-amyloid protein monoclonal antibody; after the immune dying, the extracellular beta-amyloid plaque ofthe model organism brain slice shows bright plaque in a fluorescence microscope; a fluorescence microscope is used for shooting a picture of the fluorescence microscope subjected to immunostaining; the picture is processed to recognize the located bright plaque region of the beta-amyloid plaque; through the image processing, the beta-amyloid plaque indicated by the bright plaque is subjected to automatic quantity statistics and feature calculation to obtain the statistics data; the statistics data is matched with feature information of the model organism for statistics analysis. The deposition condition of the beta-amyloid plaque is quantificationally reflected by a method of recognizing and measuring the bright plaque in the pictures shot by the fluorescence microscope.

Description

technical field [0001] The invention relates to the technical field of biological image processing, in particular to a method for identifying and measuring beta amyloid plaques based on image processing. Background technique [0002] Alzheimer's disease is the most common cause of dementia and is considered the greatest public health threat of our generation. The typical pathological feature of Alzheimer's disease is the deposition of extracellular β-amyloid plaques (Aβ plaques). Automatically and efficiently measuring characteristics such as the number of β-amyloid plaques can help study the extent and pathogenesis of Alzheimer's disease. [0003] β-amyloid protein (amyloid β-protein, Aβ), which is hydrolyzed from β-amyloid precursor protein (APP), is secreted by cells, and has a strong neurotoxic effect after the accumulation of cell matrix precipitation . It can be produced by a variety of cells and circulates in the blood, cerebrospinal fluid and interstitial fluid of...

AI Technical Summary

Problems solved by technology

The traditional manual counting method has many limitations: firstly, it is very time-consuming to artificially count hundreds of β-amyloid plaques in each picture, and the subjective judgment of different people is also different; secondly, manual measurement can only Counting the number of β-amyloid plaques cannot give accurate measurements for other characteristics, such as size, distribution and other characteristics; in addition, the increasing large-scale experiments are too inefficient only by manual measurement

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