Glioma diagnostic marker circ6:22020339|22020542 and its application
A technology for glioma and exosomes, applied in biochemical equipment and methods, microbial measurement/testing, etc., can solve the problems of poor postoperative survival rate, poor treatment effect, high recurrence rate, etc., and achieve fast and accurate The effect of mastering the patient's condition, high diagnostic and prognostic analysis value, and convenient and accurate diagnosis
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[0019] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.
[0020] 1. Research object
[0021] 1. The two glioma cell lines are U251 and U87 cell lines, and the six primary glioma cell lines are 1216, 1125, 1124B, 1124C, 0128C, and 1104, all provided by the Institute of Cancer, School of Basic Medicine, Central South University. 2. The serum samples of 40 glioma patients were provided by Xiangya Hospital, and the 15 normal serum samples were healthy individuals who underwent community disease screening at the same time. The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.
[0022] 2. Research methods
[0023] 1. Extraction of RNA from cells, exosomes secreted by cells, and glioma / normal serum exosomes
[0024] A. Extraction of cellular RNA
[0025] After the above 8 kinds of cells grow to an appropriate density, disc...
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