Vital algae quick detection device for ballast water and detection method of vital algae quick detection device

Abstract

The invention discloses a vital algae quick detection device for ballast water and a detection method of the vital algae quick detection device, relates to the technical field of detectors, and aims to solve the technical problem on detection of the activity of algae in the ballast water. The device comprises a sample chamber, wherein a sample basin is arranged in the sample chamber; an excitationlight source is arranged on each of the left and right sides of the sample chamber; emergent light axes of the two excitation light sources are intersected at the sample basin; one of the excitationlight source is a pulse excitation light source, and the other excitation light source is a saturated excitation light source; the photon flux density of emergent light rays of the pulse excitation light source is 0.1 to 1 micromole photon per square second; the photon flux density of emergent light rays of the saturated excitation light source is 2,000 to 4,000 micromole photon per square second;a photoelectric receiver for receiving the emergent light rays of the sample chamber is arranged in front of a light emergent window of the sample chamber. The device and the method which are provided by the invention can quickly detect the activity of the algae in the ballast water.

Description

technical field [0001] The invention relates to detection instrument technology, in particular to a technology for quickly detecting live algae in ballast water and a detection method thereof. Background technique [0002] The damage to the marine environment by marine species carried by ships' ballast water is one of the four major hazards of the ocean. It is estimated that about 10 billion tons of ballast water are transferred around the world every year, and more than 3,000 species of organisms migrate around the world through ballast water every day, and most of the marine plankton they carry are in the form of larvae About 5%-10% of the species introduced into the new environment can grow locally. To this end, the International Maritime Organization (IMO) adopted the International Convention on the Management and Control of Ships' Ballast Water and Sediments in London on February 13, 2004, in order to better manage and control the discharge of ballast water. The D-2 s...

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Problems solved by technology

[0004] Aiming at the rapid and simple detection of the number of 10um~50um living microalgae in ballast water, there are many kinds of devices, most of which still use fluorescent staining to distinguish the life and death of microalgae, and use flow cytometric conductance or laser-induced fluorescence counting to realize automatic detection. Counting saves the workload of manual microscopic inspection and counting, and can give specific values, but it still needs to use dyes, which will cause secondary pollution, and automatic counting is more suitable for counting of higher quantities, and the number of microalgae is required to be as low as For ballast water less than 10 / mL, a certain proportion of concentration treatment is required, and the operation process is still complicated

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