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A method for separating and purifying L-hydroxyproline from L-hydroxyproline fermentation broth

A hydroxyproline, separation and purification technology, applied in the directions of organic chemistry, organic chemistry, etc., can solve the problems of high energy consumption, increased energy consumption, low total yield, etc. The effect of reducing power cost and energy consumption and improving crystallization yield

Active Publication Date: 2020-06-16
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process route does not pretreat the fermentation liquid, the three membrane filtrations consume a lot of energy, and the volume of the feed liquid increases step by step, resulting in a large energy consumption in the concentration process, further increased energy consumption in the recrystallization process, and a low overall yield.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for separating and purifying L-hydroxyproline from L-hydroxyproline fermentation broth, specifically as follows:

[0036]Take 100L of L-hydroxyproline fermentation broth (L-hydroxyproline content 50.5g / L) and adjust the pH to 4.0 with 50% sulfuric acid, add 0.5% aluminum potassium sulfate, keep at 60°C for 30 minutes, and then filter with a 50nm ceramic membrane Sterilize to obtain 120L of L-hydroxyproline filtered supernatant (L-hydroxyproline content is 41.25g / L); the supernatant is passed through an ultrafiltration membrane with a molecular weight cut-off of 6000D to obtain 145L of filtrated supernatant (with a content of 33.45g / L); add 10% (w / w) activated carbon (485g) to the ultrafiltrate, 0.5% (w / w) (24.3g diatomite), heat it at 65°C for 0.5h, and then remove the composite adsorbent by plate and frame filtration Add 0.2% or 300g of barium hydroxide and 0.01% or 14.5g of EDTA to the filtered clear liquid, keep stirring for 0.5h, and filter out the precipi...

Embodiment 2

[0038] A method for separating and purifying L-hydroxyproline from L-hydroxyproline fermentation broth, specifically as follows:

[0039] Take 120L of L-hydroxyproline fermentation broth (L-hydroxyproline content 56.8g / L) and adjust the pH to 3.5 with 50% sulfuric acid, add 0.5% ferric chloride, keep it at 65°C for 30min, and use a 30nm organic tube 145L L-hydroxyproline filtered supernatant (L-hydroxyproline content is 46.54g / L); the supernatant is passed through an ultrafiltration membrane with a molecular weight cut-off of 10000D to obtain a filtered supernatant of 170L (L-Hydroxyproline content 39.3g / L); add 15% (w / w) activated carbon, namely 1.0kg, 0.5% (w / w), namely 33.4g diatomaceous earth, to the ultrafiltrate, 70°C After 0.5h of heat preservation, the composite adsorbent was removed by plate and frame filtration; 0.5% (w / v) or 850g of barium hydroxide and 0.01% (w / v) of 17g of EDTA were added to the filtered liquid, and the plate and frame were kept stirring for 0.5h ...

Embodiment 3

[0041] A method for separating and purifying L-hydroxyproline from L-hydroxyproline fermentation broth, specifically as follows:

[0042] Take 150L of L-hydroxyproline fermentation broth (L-hydroxyproline content 48.5g / L) and adjust the pH to 4.0 with 50% sulfuric acid, add 1.0% ammonium aluminum sulfate, incubate at 70°C for 30 minutes and filter with a 50nm ceramic membrane Sterilize to obtain 180L of L-hydroxyproline filtered supernatant (the L-hydroxyproline content is 40g / L); Amino acid content 33.61g / L); add 20% (w / w) activated carbon to the ultrafiltrate, namely 1.4kg, 0.7% (w / w), namely 49.4g of diatomite, heat at 70°C for 0.5h and then plate Remove the composite adsorbent by frame filtration; add 0.4% (w / v) or 840 g of barium chloride and 0.02% (w / v) or 42 g of EDTA to the filtered clear liquid, keep stirring for 0.5 h, and remove the precipitate by plate and frame filtration; 230L of the liquid (L-hydroxyproline content 29.46g / L) was concentrated in vacuum at 50°C u...

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Abstract

The invention belongs to the field of biochemical engineering and specifically relates to a method for separating and purifying L-hydroxyproline from L-hydroxyproline fermentation liquor. The method comprises the following steps: separating bacterial solution of the fermentation liquor, filtering with an ultrafiltration membrane, treating with a compound absorbent, desalting and performing vacuum concentration crystallization. According to the method provided by the invention, the fermentation liquor is pretreated and then is filtered for removing bacteria, so that the filtration flux is increased, the filtering time is shortened and the removal rate of protein is 20% or above; no cation exchange resin is used and the acid-alkali wastewater is greatly reduced; the nanofiltration membrane or reverse osmosis membrane treatment is not required, the qualified product is acquired through primary crystallization, the power cost and energy consumption are greatly reduced and an efficient low-cost method for separating and purifying hydroxyproline is formed; according to the invention, the production process route is simplified, the production period is shortened, the production cost is lowered, the extraction rate of the L-hydroxyproline is increased to 85% or above and the content of the end product is 99.0% or above.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a fenlichujnhua method for L-hydroxyproline. Background technique [0002] L-Hydroxyproline is an imino acid, which is the product of hydroxylation of proline and the main component of collagen, and does not belong to the 20 common amino acids. In recent years, the research and development of L-Hyp has attracted widespread attention, and it is used in medicine, chemical industry, animal feed, nutrition and beauty industries, etc. [0003] At present, the methods for synthesizing L-hydroxyproline include hydrolysis extraction, biological enzyme method and direct fermentation method. The production of L-hydroxyproline in my country mainly adopts the chemical hydrolysis extraction process, which uses animal collagen as raw material, and is prepared through strong acid hydrolysis, nitrous acid oxidation and ion exchange. The yield is only 5%, and the discharge of "three wastes" i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D207/16
CPCC07B2200/07C07D207/16
Inventor 郭小雷黄建忠吴伟斌翁成光
Owner FUJIAN NORMAL UNIV
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