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A kind of adjuvant composition and its preparation method and application

A technology of composition and adjuvant, applied in the field of adjuvant composition and preparation thereof, can solve the problems of no adjuvant effect and the like

Active Publication Date: 2021-01-01
LUOYANG SEIWEI BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prior art does not have the report that it has adjuvant effect

Method used

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  • A kind of adjuvant composition and its preparation method and application
  • A kind of adjuvant composition and its preparation method and application
  • A kind of adjuvant composition and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of an adjuvant composition containing polyacrylic acid-ε-polylysine nanoparticles

[0035] Dissolve polyacrylic acid (commercially available Carbomer 934P) and polylysine in 0.85% NaCl solution, and adjust the pH thereof to 7.2 to obtain polyacrylic acid solution and polylysine solution. The polyacrylic acid solution and the polylysine solution are mixed under stirring conditions to obtain an adjuvant composition containing polyacrylic acid-ε-polylysine nanoparticles with negative charges on the surface.

[0036] The particle size of the formed nanoparticles was measured using DLS (Dynamic Light Scattering), showing that the formed nanoparticles had a particle size of 200-1500 nm. See Table 1 for the formulation of the adjuvant composition.

[0037] Table 1: Formulation Table of Adjuvant Compositions

[0038] group Carbomer 934P Polylysine Sodium chloride immune booster water 1 (Adjuvant 1) 0.5g 0.5g 0.85g — Add to ...

Embodiment 2

[0039] Embodiment 2: the promoting action of adjuvant composition of the present invention to porcine circovirus to produce neutralizing antibody

[0040] 2.1 Preparation of vaccine composition

[0041] To 50 g of the adjuvant composition prepared in Example 1, 20 μg / head of PCV2 ORF2 protein was added, and the volume was constant with phosphate buffer at pH=7.4 to obtain a vaccine composition. The obtained vaccine composition was placed at 4°C to observe its stability, and the results are shown in Table 2.

[0042] Table 2: Formulation and Stability of Vaccine Compositions

[0043]

[0044]

[0045] It can be seen from Table 2 that the vaccine composition prepared by using the adjuvant composition of the present invention has high stability and can be kept stable for at least 18 months at 4°C.

[0046] 2.2 Neutralizing antibody titer experiment

[0047] Thirty 30-day-old PCV2-negative healthy piglets were randomly divided into 6 groups with 5 piglets in each group. ...

Embodiment 3

[0067] Embodiment 3: The protection test of the adjuvant composition of the present invention to Mycoplasma hyopneumoniae

[0068] 3.1 Preparation of vaccine composition

[0069] In 50g of the adjuvant composition prepared in Example 1, add 2×10 8 / part of Mycoplasma antigen 50g to obtain the vaccine composition. The obtained vaccine composition was placed at 4°C to observe its stability, and the results are shown in Table 7.

[0070] Table 7: Formulation and Stability of Vaccine Compositions

[0071]

[0072]

[0073] 3.2 Test piglets immunized with vaccines

[0074] 30 piglets aged 14-21 days were selected and randomly divided into 6 groups, 5 piglets / group. The vaccine composition I, II, III, IV prepared in 3.1 and the comparison vaccine were respectively injected into the piglets of the 1st to 5th groups, and each piglet was injected with 1 ml of the corresponding inactivated vaccine in the neck muscle respectively; the 6th group was injected with the same amoun...

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Abstract

The invention relates to the technical field of animal biological products, in particular to an adjuvant composition and a preparation method and application thereof. The adjuvant composition comprises acrylic acid polymer-epsilon-polylysine nano-particles; and specifically, 100 parts by weight of the adjuvant composition comprises 0.001 to 5 parts by weight of acrylic acid polymer-epsilon-polylysine nano-particles. The acrylic acid polymer-epsilon-polylysine nano-particles contained in the adjuvant composition are prepared through a simple electrostatic interaction, and the adjuvant composition is free of cross-linking among proteins harmful for bodies, so that the adjuvant composition has extremely high safety and effectiveness, is applicable to be used as an adjuvant, and overcomes problems existing when polyacrylic acid is used as the adjuvant. Moreover, a vaccine composition formed by the adjuvant composition and an antigen has extremely high stability for long-term storage, and can be safely and effectively applied to various types of subjects.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to an adjuvant composition and its preparation method and application. Background technique [0002] Adjuvants are materials used to produce vaccines with increased antigenicity or for therapeutic and prophylactic purposes by increasing nonspecific immune responses to antigens. When the antigen level is low, adjuvants are used to maintain a strong and rapid immune response against the antigen for a longer period of time, and these adjuvants can be used in the preparation of vaccines. Adjuvants can use specific antigens or can change the level of antigens, thereby modulating the immune response to antigens or controlling the type and subclass of antibodies directed against antigens. In addition, adjuvants can also be used to enhance the immune response, especially for immune immature or aging people, to enhance the induction of mucosal immunity. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/00A61K39/02A61P31/04
CPCA61K39/00A61K39/0208A61K39/39A61K2039/52A61K2039/55511
Inventor 田克恭刘永梅张许科
Owner LUOYANG SEIWEI BIOTECHNOLOGIES CO LTD
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