Three-dimensional artificial total intervertebral disc as well as preparation method and applications thereof

A technology of full intervertebral disc and intervertebral disc, which is applied in the field of three-dimensional artificial full intervertebral disc, can solve problems such as uncontrollability of electrospinning jet, and achieve the effect of good biocompatibility

Inactive Publication Date: 2017-12-22
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the uncontrollability of the electrospinning jet, most of the nanofiber scaffolds prepared by traditional methods are in the form of non-woven fabrics with random arrangement of fiber directions. Anisotropic fibrous annulus composed of ordered arrays remains a challenging problem

Method used

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  • Three-dimensional artificial total intervertebral disc as well as preparation method and applications thereof
  • Three-dimensional artificial total intervertebral disc as well as preparation method and applications thereof
  • Three-dimensional artificial total intervertebral disc as well as preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] This example is used to illustrate the preparation method of the PPC-I intervertebral disc of the present invention.

[0073] According to the mass ratio of PCL: PLGA: Collagen of 4: 2: 1, 1 g was dissolved in 10 mL of hexafluoroisopropanol to prepare 10% pre-spinning solution, and then spun according to the method of Example 1 to prepare ordered and disordered PPC -I spinning. Spinning was performed at a 17G needle with a voltage of 15kV and a liquid feeding speed of 1mL h-1. We use a static collection device to prepare random spinning, using a line speed of 13 m s -1 High-speed rotating roller collection device to prepare orderly spinning. Then cut the ordered spinning obtained according to the above steps into strips, so that the spinning arrangement direction and the long axis of the strips are + / -30° long strips, and then the +30° and -30° two-layer spinning The silk films are stacked and then rolled up around a circular shaft to form a multi-layer concentric ri...

Embodiment 2

[0076] This example is used to illustrate the preparation method of the PPC-II intervertebral disc of the present invention.

[0077] According to the mass ratio of PCL: PLGA: Collagen of 2: 2: 1, 1 g was dissolved in 10 mL of hexafluoroisopropanol to prepare 10% pre-spinning liquid, and then spinning according to the method of Example 1 to prepare ordered and disordered PPC -II spinning. Apply voltage 15kV to 17G needle, 1mL h -1 The liquid feeding speed is used for spinning. We use a static collection device to prepare random spinning, using a line speed of 13 m s -1 High-speed rotating roller collection device to prepare orderly spinning. Then cut the ordered spinning obtained according to the above steps into strips, so that the spinning arrangement direction and the long axis of the strips are + / -30° long strips, and then the +30° and -30° two-layer spinning The silk films are stacked and then rolled up around a circular shaft to form a multi-layer concentric ring str...

Embodiment 3

[0080] This example is used to illustrate the preparation method of the PPC-III intervertebral disc of the present invention.

[0081] According to the mass ratio of PCL: PLGA: Collagen 3: 3: 1, 1 g was dissolved in 10 mL of hexafluoroisopropanol to prepare 10% pre-spinning solution, and then spinning according to the method of Example 1 to prepare ordered and disordered PPC -III spinning. Apply voltage 15kV to 17G needle, 1mL h -1 The liquid feeding speed is used for spinning. We use a static collection device to prepare random spinning, using a line speed of 13 m s -1High-speed rotating roller collection device to prepare orderly spinning. Then cut the ordered spinning obtained according to the above steps into strips, so that the spinning arrangement direction and the long axis of the strips are + / -30° long strips, and then the +30° and -30° two-layer spinning The silk films are stacked and then rolled up around a circular shaft to form a multi-layer concentric ring str...

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Abstract

The invention provides a three-dimensional artificial total intervertebral disc as well as a preparation method and applications of the three-dimensional artificial total intervertebral disc. According to the technical scheme, a spun membrane with high hydrophilicity, high water retention and high biocompatibility is prepared by blending PLGA, PCL and collagen. The three-dimensional artificial total intervertebral disc provided by the invention can simulate a micro-nano structure in the body, and also has good biocompatibility, the ordered nano fibers on the experimented surfaces of cells can induce directional orientation and growth of the cells, the artificial intervertebral disc implanted into the experimented surfaces of animals can be effectively combined together with surrounding tissues, so that a mechanical activity unit capable of freely moving is formed.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a three-dimensional artificial total intervertebral disc, its preparation method and application. Background technique [0002] Chronic neck and lower back pain is a disease with a very high incidence rate, and huge sums of money are spent on the treatment of diseases caused by intervertebral disc lesions every year. According to statistics, more than 97% of individuals over the age of 50 suffer from varying degrees of neck and back pain. The most common pain in the waist and neck is closely related to lesions in the intervertebral disc, mainly caused by lesions in the intervertebral disc. The current treatment for degenerative disc disease includes conservative treatment (drugs and physiotherapy) to surgical treatment (discectomy, spinal fusion and disc replacement). However, these treatments can relieve pain or restore some functions of the intervertebral disc, but canno...

Claims

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Application Information

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IPC IPC(8): A61F2/44A61L27/18A61L27/24A61L27/20A61L27/02A61L27/50
CPCA61F2/442A61F2/30942A61F2002/30963A61F2002/4495A61L27/025A61L27/18A61L27/20A61L27/24A61L27/50A61L2400/12A61L2430/38C08L67/04C08L5/04
Inventor 蒋兴宇杨俊川
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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