tRF-Gly antisense strand inhibitor and application thereof

An antisense chain and inhibitor technology, applied in the field of biomedicine, can solve problems that need further research

Active Publication Date: 2017-12-12
THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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  • Description
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Although there have been related research reports, the molecular mechanism o

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  • tRF-Gly antisense strand inhibitor and application thereof
  • tRF-Gly antisense strand inhibitor and application thereof
  • tRF-Gly antisense strand inhibitor and application thereof

Examples

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[0016] Example 1:

[0017] 1. Mouse alcoholic liver disease model and liver function index detection

[0018] C57BL / 6 mice were used, 6 in each group. The modeling process of the short-term alcohol feeding model includes: the control group is fed the non-alcoholic Lieber-DeCarli control feed; the short-term alcohol feed experimental group, the mice are first fed the non-alcoholic Lieber-DeCarli liquid feed for 5 days (adaptation period), The mice were fed with Lieber-DeCarli liquid feed containing 5% alcohol for 11 days (modeling period), and alcohol was added to the stomach (5g / kg body weight) once on the last day, and specimens were collected.

[0019] The modeling process of the long-term alcohol feeding model includes: the control group is fed the non-alcoholic Lieber-DeCarli control feed; the long-term alcoholic feed experimental group, the mice are first fed the non-alcoholic Lieber-DeCarli liquid feed for 3 days (adaptation period), Mice were fed Lieber-DeCarli liquid feed c...

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Abstract

The invention discloses a tRF-Gly antisense strand inhibitor and application thereof. In the invention, a mouse alcoholic liver disease model is used for performing deep sequencing on micro-molecules RNA in a liver tissue of an alcoholic liver disease mouse and a normal control liver tissue, and the micro-molecules RNA expressed differentially are analyzed and then are screened, so as to obtain tRF-Gly. Through methylation and cholesterol modification, by adding modification sequences at two ends, the tRF-Gly antisense strand inhibitor is chemically synthesized; through a mouse in-vivo experiment, the result indicates that the tRF-Gly antisense strand inhibitor has the advantages of effectively reducing ALT and AST and obviously reducing the accumulation of fat in liver, therefore, the tRF-Gly antisense strand inhibitor has the effect of protecting liver function and relieving an alcoholic fatty liver disease. A tRF-Gly sequence in a human body is the same as that in a mouse, therefore, the tRF-Gly antisense strand inhibitor is expected to become a novel micro-molecule RNA drug for treating the alcoholic fatty liver disease.

Description

Technical field: [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a tRF-Gly antisense chain inhibitor and application thereof. Background technique: [0002] Alcoholic liver disease (ALD) is a liver disease induced by long-term heavy alcohol intake. It manifests as alcoholic fatty liver in the early stage, and can further develop into alcoholic hepatitis, cirrhosis, and even liver cancer as the disease progresses. Alcohol abuse ranks fifth among all risk factors for disease and death, and has become a global public health problem. The incidence of ALD in my country is increasing year by year, and it has become the second leading cause of liver disease, threatening human health. At present, its pathogenesis is not fully understood, and effective treatment methods are lacking. [0003] Small non-coding RNAs are a class of endogenous non-protein-coding small RNAs of 20-30 nucleotides. Studies have found that whether it is anima...

Claims

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Application Information

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IPC IPC(8): C07H21/02A61K31/7088A61P1/16A23L33/13
CPCA23L33/13A23V2002/00C07H21/02A23V2200/30
Inventor 何松青钟伏弟江克清
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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