Application of TM9SF1 gene as target point to vascular disease
A gene and target technology, applied in the application field of vascular diseases
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Embodiment 1
[0087] 1 Materials and methods
[0088] 1.1 Cells and main reagents
[0089] HUVEC were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. TM9SF1-specific siRNA (SEQ ID NO.1) was designed and synthesized by Gemma. Endothelial cell-specific medium EGM was produced by Swiss LONZA Company; trypsin (including EDTA), fetal bovine serum (FBS), and phosphate buffered saline (PBS) were produced by American Hyclone Company; transfection reagent Lipofectamine 3000 was purchased from American Thermo Company ; CD31 antibody was purchased from Immunoway Company in the United States; Immunocytochemistry Chromogenic Kit was purchased from Beijing Zhongshan Jinqiao Company; CYBR Green Mix was purchased from Beijing Kangwei Century Biotechnology Co., Ltd.; CCK8 was purchased from Yisheng Company.
[0090] 1.2 HUVEC cell culture and identification
[0091] HUVEC were cultured in EGM, a special medium for endothelial cells, at 37.5°C, 5% CO 2 Culture in an incubator wi...
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