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Treatment method for improving wood cellulose enzymolysis saccharification

A technology of cellulase and treatment method, which is applied in the field of wood cellulose treatment and improving the treatment field of wood cellulose enzymatic hydrolysis and saccharification, which can solve the problems of high consumption cost, achieve the elimination of biological resistance, improve the efficiency of enzymatic hydrolysis, and simple process Effect

Inactive Publication Date: 2017-12-01
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the consumption cost is high and has application limitations

Method used

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  • Treatment method for improving wood cellulose enzymolysis saccharification
  • Treatment method for improving wood cellulose enzymolysis saccharification

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Dissolve 5% sodium hydroxide and 10% urea in deionized water to obtain a sodium hydroxide-urea mixture; pre-cool the mixture to -10 °C, dissolve kraft softwood bleached pulp, and add to the obtained solution The mass concentration of cellulose is 2%,; the obtained solution is left standing at -10 ℃ for 1 hour, diluted with water, and 80% of the dissolved cellulose is precipitated, the cellulose is filtered and separated through a sand core funnel, washed with water until neutral, and air-dried Prepare white, fibrous regenerated cellulose; add regenerated cellulose and cellulase to the acetic acid-sodium acetate buffer solution with a pH value of 4, the dosage of cellulase is 20 FPU / g, and the mass concentration of cellulose is 5% , and enzymatically hydrolyzed at 30°C for 11 hours to obtain a cellulose hydrolyzate rich in reducing sugars, and the saccharification rate of cellulose was measured to be 75.8%.

[0031] Set up a control sample, use the same cellulase dosage ...

Embodiment 2

[0033]Dissolve 6% sodium hydroxide and 12% urea in deionized water to obtain a sodium hydroxide-urea mixture; pre-cool the mixture to -20 °C, dissolve kraft softwood bleached pulp, and add to the obtained solution The mass concentration of cellulose is 5%,; the obtained solution is left standing at -5 °C for 2 hours, diluted with water, and 75% of the dissolved cellulose is precipitated, the cellulose is filtered and separated through a filter cloth, washed with water until neutral, and dried in an oven to obtain White, fibrous regenerated cellulose; add regenerated cellulose and cellulase to citric acid-sodium citrate buffer solution with a pH value of 4, the amount of cellulase is 30 FPU / g, and the mass concentration of cellulose is 5% , enzymatically hydrolyzed at 60°C for 15 hours to obtain a cellulose hydrolyzate rich in reducing sugars, and the saccharification rate of cellulose was measured to be 86.8%;

[0034] Set up a control sample, use the same cellulase dosage of ...

Embodiment 3

[0036] Dissolve 9% sodium hydroxide and 15% urea in deionized water to obtain a sodium hydroxide-urea mixture; pre-cool the mixture to -25°C to dissolve kraft softwood bleached pulp, and obtain a solution The mass concentration of cellulose in the medium is 6%; the obtained cellulose solution is left standing at 0°C for 3 hours, diluted with water, and 85% of the dissolved cellulose is precipitated, filtered and separated through filter paper, washed with water until neutral, and air-dried for a period of time, and then Obtain white, fibrous regenerated cellulose with oven drying; Add regenerated cellulose and cellulase in the citric acid-sodium citrate buffer solution of 5.5 pH value, enzyme consumption is 50FPU / g, and cellulose mass concentration is 7%, hydrolyzed at 50°C for 20 hours to obtain a cellulose hydrolyzate rich in reducing sugars, and the saccharification rate of cellulose was measured to be 93.4%;

[0037] Set up a control sample, use the same cellulase dosage o...

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Abstract

The invention discloses a treatment method for improving wood cellulose enzymolysis saccharification and relates to the field of enzymolysis saccharification treatment. According to the method disclosed by the invention, common wood cellulose serves as a precursor, raw materials are rich, the yield is full and can be ensured, the raw material cost is low, and the aims of saving energy and reducing cost are achieved; the process disclosed by the invention is simple, the natural crystal structure of the cellulose is changed by the processes of dissolving, regenerating and performing enzymolysis, the bio-resistance on the cellulase is eliminated, wood cellulose enzymolysis saccharification is realized by utilizing the method which is easy to operate, time-saving, energy-saving, high-efficiency and easy to realize industrialization, the cellulose is subjected to rapid enzymolysis so as to produce reducing sugar, the enzymolysis efficiency is improved, and the treatment method has important realistic significances on energy regeneration and recycling of the cellulose.

Description

technical field [0001] The invention discloses a wood cellulose treatment method, relates to the field of enzymatic saccharification treatment, in particular to a treatment method for improving enzymatic saccharification of wood cellulose. Background technique [0002] Cellulose is the main component of plant cell walls. Plants can produce about 1 trillion tons per year through photosynthesis. It is the most widely distributed and most abundant organic resource in nature, accounting for more than 50% of the carbon content in the plant kingdom. As the most abundant and cheap renewable resource in nature, cellulose is renewable and biodegradable, and can be used as an important source of fermented sugar for biomass energy production and other chemical products, replacing non-renewable fossils resource. [0003] Cellulose is a macromolecular polysaccharide formed by glucose through glycosidic bonds, and forms a certain type of crystal structure through hydrogen bonds. It is in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14C12P2201/00
Inventor 陈洪雷赵鑫刘玉孔凡功王守娟
Owner QILU UNIV OF TECH
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