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A kind of cultivating method of astragalus seedlings

A cultivation method and technology of Astragalus, applied in the directions of horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effects of reducing harmful gases, shortening the cultivation period and low production cost

Active Publication Date: 2019-08-20
INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports and related patents on cultivating Astragalus seedlings using intermittent submerged bioreactors. The present invention will disclose a new technology for the production of Astragalus seedlings

Method used

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  • A kind of cultivating method of astragalus seedlings
  • A kind of cultivating method of astragalus seedlings

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present invention is a kind of cultivation method of Astragalus seedlings, the method comprises the following steps:

[0034] (1) Preparation of explants: In a sterile operating table, cut the solid cultured Astragalus tissue culture seedlings into stem segments containing 2 to 3 leaf buds, and inoculate 100 Astragalus stem segments into the sterilized and cooled 1L Astragalus stem segments. In the triangular flask of the liquid medium, the formula of the medium is: MS+IBA0.05mg / L+6-BA0.5mg / L+sucrose 30g / L pH5.8~6.0, after the inoculation is completed, put the triangular flask into the culture room cultured for 3 days;

[0035] (2) Proliferation culture: In a sterile operating table, pour the prepared explants in step (1) together with the liquid medium into the sterilized and cooled reactor tank, and connect the tank to the reactor tank. In the device system, the immersion frequency is set to 5min / 4h, and the culture room is cultured for 15 days;

[0036](3) Root...

Embodiment 2

[0039] The present invention is a kind of cultivation method of Astragalus seedlings, the method comprises the following steps:

[0040] (1) Explant preparation: In a sterile operating table, cut the solid cultured Astragalus tissue culture seedlings into stem segments containing 2 to 3 leaf buds, and inoculate 150 Astragalus stem segments into the sterilized and cooled 1L In the triangular flask of the liquid medium, the formula of the medium is: MS+IBA0.07mg / L+6-BA0.3mg / L+sucrose 30g / L pH5.8~6.0, after the inoculation is completed, put the triangular flask into the culture room cultured for 5 days;

[0041] (2) Proliferation culture: In a sterile operating table, pour the prepared explants in step (1) together with the liquid medium into the sterilized and cooled reactor tank, and connect the tank to the reactor tank. In the device system, the immersion frequency is set to 8min / 6h, and the culture room is cultured for 20 days;

[0042] (3) Rooting culture: in the sterile o...

Embodiment 3

[0045] The present invention is a kind of cultivation method of Astragalus seedlings, the method comprises the following steps:

[0046] (1) Explant preparation: In a sterile operating table, cut the solid cultured Astragalus tissue culture seedlings into stem segments containing 2 to 3 leaf buds, and inoculate 120 Astragalus stem segments into the sterilized and cooled 1L In the triangular flask of the liquid medium, the formula of the medium is: MS+IBA0.06mg / L+6-BA0.6mg / L+sucrose 30g / L pH5.8~6.0, after the inoculation is completed, put the triangular flask into the culture room cultured for 4 days;

[0047] (2) Proliferation culture: In a sterile operating table, pour the prepared explants in step (1) together with the liquid medium into the sterilized and cooled reactor tank, and connect the tank to the reactor tank. In the device system, the immersion frequency is set to 6min / 5h, and the culture room is cultured for 18 days;

[0048] (3) Rooting culture: In the sterile o...

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Abstract

The invention provides a culture method of astragalus mongholicus germchits. The culture method is used for culturing by utilizing an intermittent submerged bioreactor; the culture method comprises the following steps: (1) preparing explants; (2) carrying out multiplication culture; (3) carrying out rooting culture. According to the culture method provided by the invention, the astragalus mongholicus germchits are cultured by utilizing an the intermittent submerged culture method for the first time; the culture method has the advantages of short germchit culture period, high germchit quality, good rooting effect and low production cost and a new technology is provided for culture and production of the astragalus mongholicus germchits.

Description

technical field [0001] The invention belongs to the field of Chinese medicinal material seedling cultivation, in particular relates to a method for culturing Astragalus seedlings, in particular to a method for culturing Astragalus seedlings by using an intermittent submerged bioreactor. Background technique [0002] Astragalus [ Astragalus membranaceus Bge. Var. mongolicus (Bge.) Hsiao] is a perennial herb of the genus Astragalus, the root of which can be used as medicine. With the increasing popularity of astragalus health medicines, the demand for astragalus is increasing. Due to long-term over-excavation, wild resources are almost depleted, and astragalus seeds have hardened phenomena, the germination rate is low, and artificial seed reproduction is also affected. Therefore, the research on tissue culture and plant regeneration of Astragalus is of great significance in the production of seedlings. The research on the tissue culture of Astragalus in my country started a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张本厚胡燕花陈集双张保钱陈国晶郝圣响
Owner INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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