Reagent for homogenizing sputum microorganism specimen

Abstract

The invention provides a method and a reagent for homogenizing a sputum specimen. The reagent is prepared from 0.2% (w / v) of N-acetyl-L-cysteine, 0.1% (w / v) of trypsin, 0.4% (w / v) of triethanolamine, 0.2% (w / v) of potassium chloride, 1.5% (w / v) of monopotassium phosphate, 0.2% (w / v) of dipotassium hydrogen phosphate and a sterile water solvent.

Description

technical field [0001] The invention relates to a method for homogenizing sputum samples and a reagent used for treating clinical sputum samples, which can effectively homogenize sputum and protect bacteria including Legionella in the sputum from being damaged , which is conducive to further analysis of sputum samples. Background technique [0002] Sputum samples collected by natural expectoration method and sputum suction method in the examination of respiratory diseases have obvious heterogeneity, and the distribution of bacteria and other components is very uneven. It is generally necessary to use sputum digestive juice to homogenize the samples . With the improvement of inspection accuracy and experimental quality control requirements, more and more attention has been paid to the pretreatment of specimens. According to daily work observations, the culture results of sputum specimens and the rate of agreement with actual clinical conditions are still not ideal, and sputu...

AI Technical Summary

Problems solved by technology

[0004] Although the above-mentioned sputum digestive juices and digestion methods have been used for the detection of sputum samples, the results of clinical practice show that these digestive juices can homogenize and protect the bacteria in the sample (especially some bacteria that are more easily inactivated in the external environment). Bacteria such as Legionella) are still lacking

Moreover, the stability of the dithiothreitol component is poor, and the storage time of the reagent is short

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