Smart glutathione sulfur transferase based on light-responsive supramolecular switch

Abstract

The invention discloses intelligent glutathione sulfurtransferase built on the basis of a photoresponse super-molecular switch. A preparation method of the intelligent glutathione sulfurtransferase includes the steps of S1, judging the catalytic site of the glutathione sulfurtransferase, substituting cysteine, and introducing cysteine at the outer edge of the combining cavity of glutathione through site-directed mutagenesis; S2, performing non-covalent compositing on azobenzene derivatives modified by cyclodextrin and maleimide to build trans-azobenzene-maleimide-cyclodextrin; S3, covalently modifying the maleimide group of the trans-azobenzene-maleimide-cyclodextrin to a cysteine mercaptan group so as to complete the specific modification of the surface of the glutathione sulfurtransferase. The preparation method has the advantages that the method is simple in design and good in catalytic and regulation effect, the closing efficiency can still reach 80.9% after 5 circulations of active opening and closing, and the method is high in practicality and free of pollution to a catalytic reaction system.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to constructing an environment-responsive intelligent enzyme system. Background technique [0002] In the process of controlling life activities under physiological conditions, enzymes are also strictly controlled by feedback loops and various trigger inductions. Inspired by the regulation of enzymes in nature, fabricating switchable artificial catalysts can precisely control biochemical reactions in biomedical applications. For example, organic catalysts containing stimuli-responsive functional groups such as azobenzene, spiropyran, and diaryl derivatives have been developed and put into use. Moreover, many ligands have also been used to modulate the catalytic activity of supramolecular catalysts based on ligand-binding-dependent allosteric control. These synthesized catalysts exhibit excellent controllability, adaptability, and change the chemical, regio, and stereoselectivi...

AI Technical Summary

Problems solved by technology

These synthesized catalysts exhibit excellent controllability, adaptability, and change the chemical, regio, and stereoselectivity of reactants or products by precisely adjusting the active sites, although the synthesized catalysts can regulate their activity through allosterism, However, the molecular efficiency of the catalyst is generally low

There is also a light regulation method to achieve enzymatic activity is to use chemically synthesized photoinhibitor molecules to competitively inhibit the substrate binding of enzymes through their light-responsive conformational changes, thereby reversibly controlling the activity of enzyme molecules, but the generality of this method Poor, can only design its unique inhibitors for a specific enzyme molecule, so the scope of application is narrow

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