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Artificial spawning and insemination method for hyriopsis cumingii

A technology for artificial eutrophication of triangular sail mussels, applied in fish farming, application, climate change adaptation, etc., can solve problems such as difficulty in family pedigree, complicated fertilization process, and injury to experimental animals, so as to improve the efficiency of oxytocin and the proportion of mature sperm, Realize the effect of manual regulation and shorten the duration

Active Publication Date: 2013-06-05
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the in vitro fertilization of seawater shellfish, physical (such as temperature, drying in the shade, water stimulation), chemical methods (such as ammonia) are usually used to induce or dissect to obtain mature sperm and eggs respectively, and after mixing, fertilized eggs are obtained and polyploidy is carried out. Breeding research is relatively easy to implement, but causes serious injury or even death to experimental animals
In addition, family selection is one of the important and effective technical methods in the research of genetic breeding. Due to the asynchronous development of the parents of S. sinensis and the inability to be fertilized at the same time, it is difficult to establish the same batch of families.
[0005] Different from seawater shellfish, the triangular sail clam has the typical reproductive characteristics of mussels: the sperm of the male clam and the eggs of the female clam need to be fertilized and developed on the external gill filaments of the female clam, and the fertilization process is more complicated
Due to weak research on basic reproductive biology, it is currently impossible to artificially control the fertilization process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. On March 10, 2010, 1,200 parents of Trigonidae mussels were collected from the Gonghu section of Taihu Lake, Jiangsu Province, and 290 individuals with 4 to 6-year-old parents were selected, and the shells were intact, strong water spray, and healthy. Through observation Gill filaments were used to differentiate male and female, and 157 female and 133 male mussels were selected.

[0022] 2. Use a cutting plotter to mark the surface of the selected male and female parental shells with Arabic numerals and letters, and then put them into small-scale cultivation ponds to strengthen cultivation. The area of ​​each cultivation pond is 2.5 square meters. The stocking density of female mussels and male mussels Both are 8 per square meter, the height of each cultivation pond is 25 cm, the water level of the cultivation pond is controlled at 10-15 centimeters, the artesian water in the high-level pond is used as the water source to keep the cultivation pond as flowing water, an...

Embodiment 2

[0028] 1. On March 10, 2010, 1,200 parents of Trigonopsis sinensis were collected from the Gonghu section of Taihu Lake, Jiangsu Province, and 290 individuals with 4 to 6-year-old parents with intact shells, strong water spray and healthy physique were selected from them. Through observation Gill filaments were used to differentiate male and female, and 157 female and 133 male mussels were selected.

[0029] 2. Use a cutting plotter to mark the surface of the selected male and female parental shells with Arabic numerals and letters, and then put them into small-scale cultivation ponds to strengthen cultivation. The stocking density of female and male mussels is 10 per square meter. The height of the pond is 25 centimeters, and the water level of each cultivation pond is 10 centimeters, and the area of ​​each cultivation pond is 2.5 square meters. Utilize the artesian water in the high-level pond as the water source to keep the cultivation pond as flowing water, and the flow rat...

Embodiment 3

[0035] 1. On March 15, 2011, 255 individuals with intact shells, strong water spray and healthy physique were selected from the parent pond of Taihu Lake Triangle clam in the Jiangyin Experimental Base of the Freshwater Fishery Research Center of the Chinese Academy of Fishery Sciences. According to the 2010 Shell surface markings, including 138 female mussels and 117 male mussels.

[0036] 2, put the selected male and female parents into small-scale cultivation ponds respectively to strengthen cultivation, the stocking density of female mussels and male clams is 5 / square meter, the height of each cultivation pond is 25 centimeters, and the water level of each cultivation pond is 15 cm. centimeter, the area of ​​each cultivation pond is 2.5 square meters, utilizes the artesian water of the elevated pool as the water source to keep the cultivation pond as flowing water, and cultivates 3 meters / minute of the flowing water in the pond.

[0037] 3. Cultivate in isolated running wa...

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Abstract

The invention discloses an artificial spawning and insemination method for hyriopsis cumingii, which includes the steps: selecting parents 4-6 years old and identifying sex; isolating female parents and male parents for synchronous cultivation in a culture pond with micro-flow water; sorting out female clams to be pregnant; dividing the female clams to be pregnant into a natural insemination group and an artificial insemination group; injecting male clams with oxytocin; and performing artificial insemination to the male clams. By the method, artificial regulation of insemination of hyriopsis cumingii is realized, artificial insemination rate of hyriopsis cumingii can reach 70%-90% in 10-30 minutes, and basic conditions for developing researches on hyriopsis cumingii, such as polyploid breeding, family selection and the like are created.

Description

technical field [0001] The invention belongs to the technical field of shellfish artificial propagation, and specifically discloses a method for artificially inducing labor and insemination of the triangular sail mussel. Background technique [0002] Triangle clams ( Hyriopsis cumuingii ) belongs to the phylum Molluscs, Bivalvia, Euphyllobranches, and Mussels. It is an endemic species in my country and one of the high-quality varieties of freshwater pearls in China. Since the 1980s, the triangular sail clam has replaced the pleated crested clam ( Cristaria plicata ), Anodonta dorsalis ( Anodonta woodiana woodiana ) and other varieties are widely used in my country's freshwater pearl industry, which makes my country's freshwater pearl production surpass Japan in one fell swoop, becoming the world's largest producer and exporter of freshwater pearls, and has continued to this day. Statistics from the China Jewelry Association show that the annual output of freshwater pear...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 华丹顾若波闻海波徐钢春聂志娟周鑫
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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