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Akermans myxobacteria culture medium and preparation method thereof

A technology of culture medium and myxobacteria, which is applied in the field of microbial engineering, can solve the problems of slow growth of Ackermann's myxobacteria, achieve the effect of shortening the culture time and promoting growth

Active Publication Date: 2020-06-16
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a kind of Akerman's myxobacteria culture medium and preparation method thereof, to solve the problem that Akerman's myxobacteria grows slowly, by screening growth stimulating factor, make culture medium can significantly improve Ackerman's Growth rate and bacterial concentration of myxobacteria

Method used

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  • Akermans myxobacteria culture medium and preparation method thereof
  • Akermans myxobacteria culture medium and preparation method thereof
  • Akermans myxobacteria culture medium and preparation method thereof

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Embodiment 1

[0034] A kind of Akermann's myxobacterium culture medium, comprises:

[0035]

[0036]

[0037] in,

[0038] Acid trace solutions include:

[0039]

[0040] Alkaline trace solutions include:

[0041]

[0042] Vitamin solutions include:

[0043]

[0044]

[0045] In the culture medium, except for the above components, the balance is water.

[0046] A preparation method for Ackermann's myxobacteria medium, comprising the following steps:

[0047] Step 1. Add a certain amount of distilled water (which can dissolve the components added later and not exceed the total amount of water, so it can be more or less) into the mixer;

[0048] Step 2, adding each component of the basal medium to distilled water at room temperature for mixing;

[0049] Step 3, adding each component of the growth stimulating factor to distilled water at room temperature for mixing;

[0050] Step 4. Add distilled water to the mixer until each component reaches the set concentration.

...

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Abstract

The invention discloses an Ackerman myxobacteria culture medium and a preparation method thereof. The Ackerman myxobacteria culture medium per liter is prepared from the following raw materials: 0.4g of monopotassium phosphate, 0.53g of disodium hydrogen phosphate, 0.3g of ammonium chloride, 0.3g of sodium chloride, 0.11g of calcium chloride, 0.1g of magnesium chloride hexahydrate, 4g of sodium bicarbonate, 0.5mg of resazurin, 1mL of an acid trace solution, 1mL of an alkali trace solution, 1mL of a vitamin solution, 37g of a brain heart infusion, 1.5g of xylooligosaccharide, 5g of cyclodextrin and 30g of solarbio protease-enzymolyzed egg white. The culture medium is capable of solving the problem of slow growth of Ackerman myxobacteria, and significantly improving the growth rate and the bacteria solution concentration of Ackerman myxobacteria.

Description

technical field [0001] The invention relates to the field of microbial engineering. More specifically, the present invention relates to a culture medium of Akermansia myxobacteria and a preparation method thereof. Background technique [0002] Akkermansia muciniphila, also known as Akkermansia muciniphila, is a Gram-negative, strictly anaerobic bacterium found in the human gut. Many studies in recent years have shown that Akkermansia has a certain relationship with many metabolic diseases in humans. [0003] Akkermansia myxobacteria has certain preventive and control effects on obesity and type 2 diabetes, and its abundance is negatively correlated with human body weight, type 1 diabetes and type 2 diabetes. [0004] Akkermansia reduced diet-induced weight gain and fat mass formation, while inversely correlated with waist circumference, body weight, and body mass index, suggesting a link between Akkermansia and body weight loss. At the same time, myxobacteria Akkermannii ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 逄晓阳吕加平提盼盼刘鹭芦晶张书文
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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