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Monodus subterraneus separation and purification method and device

A purification method and technology of unicellular algae, applied in the direction of unicellular algae, separation of microorganisms, microorganism-based methods, etc., can solve the problems of low efficiency and low success rate, improve work efficiency, simple and easy operation, convenient and neat effect of arrangement

Pending Publication Date: 2017-11-24
中国水产科学研究院南海水产研究所热带水产研究开发中心 +1
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Problems solved by technology

[0004] Aiming at the problems of low success rate and low efficiency of the existing water droplet separation method, the present invention proposes a single cell algae separation and purification method that can improve the success rate and work efficiency of the water droplet separation method

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  • Monodus subterraneus separation and purification method and device

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Embodiment Construction

[0017] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0018] like figure 1 As shown, the unit cell algae separation and purification device of the present invention includes a slide glass 1, 3 plexiglass strips 2 and 6 cover glasses 3. Wherein, the length, width and height of slide glass 1 are 7.5cm×2.5cm×0.1cm respectively, and the length, width and height of organic glass strip 2 are 2.5cm×0.5cm×0.4cm respectively. Three plexiglass strips 2 are arranged side by side on the slide glass 1 with a certain distance between each other to form two grooves. The parts of length, width and height respectively 2.5 cm×0.2 cm×0.1 cm are removed from the tops of the opposite sides of the two opposing plexiglass strips 2 to form a pallet 21 . The distance between the bottoms of two adjacent plexiglass strips 2 is 0.6cm, the side length of the cover glass 3 is 0.8cm×0.8cm, and the 6 cover glasses 3 are divided...

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Abstract

The invention relates to a monodus subterraneus separation and purification method and device. The method comprises the following steps that (1) the concentration of monodus subterraneus in a water sample is regulated, so that only one monodus subterraneus is contained in each drop of water sample; (2) each drop of water sample is singly dropped on a small-specification bearing sheet which can be directly put into a culture test tube; after microscopical examination selection, the selected bearing sheet is directly put into the culture test tube to be cultured. The method has the advantages that each drop of water sample is singly dropped on the small-specification bearing sheet which can be directly put into the culture test tube; after the microscopical examination selection, the selected bearing sheet is directly put into the culture test tube to be cultured; the problems of work efficiency influence and low success probability during the target monodus subterraneus transfer are avoided; the work efficiency of monodus subterraneus separation and purification can be greatly improved on the whole.

Description

technical field [0001] The invention relates to the field of aquatic organism bait, in particular to the field of separation and purification of unicellular algae. Background technique [0002] As an important biological bait and experimental material for many aquatic animals, unicellular algae has been successfully artificially cultivated on a large scale. However, pollution will inevitably occur during the conservation and inoculation of unicellular algae. Therefore, it is necessary to separate and purify the algae or extract them from natural water bodies. to re-isolate the target algae. The traditional separation methods of Unicellular algae include microtube separation method, water drop separation method, plate separation method, dilution method and antibiotic separation method. The principle of the micropipe separation method is to use the capillary action of the micropipette to suck the desired algae cells into the pipette at the moment the micropipette enters the w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C12N1/12C12M1/00C12R1/89
CPCC12M25/00C12N1/02C12N1/12
Inventor 王雨邓正华陈明强李有宁于刚王祎哲
Owner 中国水产科学研究院南海水产研究所热带水产研究开发中心
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