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A method for fixing and dehydrating pathological specimens

A technique for specimen fixation and processing, applied in sampling, instruments, measuring devices, etc., can solve the problems of insufficient wax dipping, incompleteness, deformation and dehydration of specimen tissues, etc., and achieve the effect of uniform wax dipping, guaranteed purity, and easy production

Active Publication Date: 2020-04-24
CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention solves the problems of pathological specimens in the prior art during fixation and dehydration treatment, specimen tissue deformation or incomplete dehydration, insufficient wax immersion, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for fixing and dehydrating a pathological specimen, comprising the following steps:

[0018] (1) Tissue fixation of pathological specimens: place the pathological specimens in a special fixative tank on the material collection platform and soak them in the fixative solution. The soaking time is determined according to the nature of the tissue of the pathological specimens;

[0019] (2) Washing of pathological specimen tissue: wash the pathological specimen tissue with tap water, and use a small amount of tap water when rinsing. the purity of the liquid;

[0020] (3) Dehydration of pathological specimen tissue: the pathological specimen tissue is dehydrated by an automatic dehydrator, and successively replaced step by step with fixative solution, AF solution, and ethanol gradient solution. The AF solution is 10% formaldehyde alcohol reagent, and the ethanol gradient solution The volume ratio of ethanol in each gradient is 95%→95%→95%→100%→100%→100%, and each pa...

Embodiment 2

[0027] A method for fixing and dehydrating a pathological specimen, comprising the following steps:

[0028] (1) Tissue fixation of pathological specimens: place the pathological specimens in a special fixative tank on the material collection platform and soak them in the fixative solution. The soaking time is determined according to the nature of the tissue of the pathological specimens;

[0029] (2) Washing of pathological specimen tissue: wash the pathological specimen tissue with tap water, and use a small amount of tap water when rinsing. the purity of the liquid;

[0030] (3) Dehydration of pathological specimen tissue: use an automatic dehydrator to dehydrate pathological specimen tissue, and fill in the instrument use record form, and replace it with fixative solution, AF solution, and ethanol gradient solution step by step. The AF solution is 10% formaldehyde alcohol reagent. , the volume ratio of ethanol in each gradient of the ethanol gradient solution is 95% → 95%...

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PUM

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Abstract

The invention discloses a pathological specimen fixing and dewatering treatment method, which comprises: fixing a pathological specimen tissue, rinsing the pathological specimen tissue, dewatering the pathological specimen tissue, permeating the pathological specimen tissue, and immersing the pathological specimen tissue into wax. According to the present invention, the pathological specimen tissue is firstly fixed with the fixing liquid and then is cleaned with tap water, such that blood stains, impure fixing liquid and other impurities can be effectively removed from the tissus, and the purity of the fixing liquid in the dewatering machine can be ensured; the gradient dewatering is used in the dewatering treatment so as to completely dewater; and the gradient permeating is performed with the xylene solution before the immersing with the wax, such that the immersing with the wax can be effectively performed, the immersing is uniform, and the late-stage slice production can be easily achieved.

Description

technical field [0001] The invention belongs to the technical field of making pathological specimens, and in particular relates to a convenient specimen fixing and dehydration treatment method. Background technique [0002] In the process of supporting pathological specimens, tissue fixation and dehydration are the key steps. Only good fixation and dehydration can ensure the smooth progress of specimen preparation. In the existing technology, almost all pathology departments routinely use tissue automatic dehydrators, but sometimes due to power failure Or the abnormality of the computer program will cause the timing function of the dehydrator to be disordered or malfunction. specimen, it would be fatally damaged. Therefore, using an automatic dehydrator to dehydrate pathological specimens is not foolproof. Therefore, how to produce high-quality pathological slides should be further optimized in the process of fixing and dehydrating pathological specimens. Contents of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/30G01N2001/305
Inventor 易达李慧源张亮商亚彤方皓
Owner CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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