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Method for establishing early metastasis model of esophageal squamous carcinoma

A technology for esophageal squamous cell carcinoma and metastasis model, which is applied in the field of biomedicine, can solve the problems such as the inability to effectively study the rules and characteristics of esophageal squamous cell carcinoma metastasis, and less detection of lymph node metastasis, so as to facilitate the construction and increase the spread.

Inactive Publication Date: 2017-11-21
ZHENGZHOU UNIV
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Problems solved by technology

[0003] In the prior art, when preparing the metastasis model of esophageal squamous cell carcinoma, the method of injecting esophageal squamous cell carcinoma cells into the tail vein or intraperitoneal cavity is used to inoculate the esophageal squamous cell carcinoma cells into the mice, and the esophageal squamous cell carcinoma cells mostly produce lung, Liver and peritoneal metastasis, and lymph node metastasis is rarely found, and clinical metastasis is mostly distant lymph node metastasis. Therefore, this kind of experimental metastasis is far from clinical metastasis of esophageal squamous cell carcinoma (mostly distant lymph node metastasis), so it cannot be effective The law and characteristics of clinical metastasis of esophageal squamous cell carcinoma

Method used

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  • Method for establishing early metastasis model of esophageal squamous carcinoma
  • Method for establishing early metastasis model of esophageal squamous carcinoma
  • Method for establishing early metastasis model of esophageal squamous carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] S101: The transwell invasion assay was used to treat esophageal squamous cell carcinoma cells with chemotaxis, and the esophageal squamous cell carcinoma cells in the middle and lower chambers of the transwell chamber were collected; the permeable membrane between the upper and lower chambers in the transwell chamber was polycarbonate membrane; Matrigel is laid on the side of the carbonate membrane facing the upper chamber; the culture medium in the upper chamber of the transwell chamber is the 1640 medium of Beijing Suo Laibao Technology Co., Ltd., and the culture medium in the lower chamber is the culture medium containing fetal bovine serum, containing The mass fraction of fetal bovine serum in the culture solution of fetal bovine serum is 20%;

[0054] S102: culturing and screening the collected esophageal squamous cell carcinoma cells to obtain esophageal squamous cell carcinoma subline cells;

[0055] S103: Repeat S101-S102 three times to obtain esophageal squamou...

Embodiment 3

[0075] S101: The transwell invasion assay was used to treat esophageal squamous cell carcinoma cells with chemotaxis, and the esophageal squamous cell carcinoma cells in the middle and lower chambers of the transwell chamber were collected; the permeable membrane between the upper and lower chambers in the transwell chamber was polycarbonate membrane; Matrigel is laid on the side of the carbonate membrane facing the upper chamber; the culture medium in the upper chamber of the transwell chamber is the 1640 medium of Beijing Suo Laibao Technology Co., Ltd., and the culture medium in the lower chamber is the culture medium containing fetal bovine serum, containing The mass fraction of fetal bovine serum in the culture solution of fetal bovine serum is 30%;

[0076] S102: culturing and screening the collected esophageal squamous cell carcinoma cells to obtain esophageal squamous cell carcinoma subline cells;

[0077] S103: Repeat S101-S103 three times to obtain esophageal squamou...

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Abstract

The invention provides a method for establishing an early metastasis model of esophageal squamous carcinoma, and belongs to the field of biomedicine. The method can effectively study the clinical regularity and characteristics of esophageal squamous carcinoma metastasis according to the early metastasis model of esophageal squamous carcinoma. The establishing method includes the steps of screening esophageal squamous carcinoma cells to obtain the subline cells of esophageal squamous carcinoma; inoculating the subline cells of esophageal squamous carcinoma to the inside of the renal capsule of a mouse to make the subline cells of esophageal squamous carcinoma grow under the renal capsule of the mouse, and obtaining the early metastasis model of esophageal squamous carcinoma. The method for establishing the early metastasis model of esophageal squamous carcinoma is used for studying the clinical regularity and characteristics of esophageal squamous carcinoma metastasis.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for establishing an early metastasis model of esophageal squamous cell carcinoma. Background technique [0002] Esophageal squamous cell carcinoma metastasis model is an animal model of esophageal cancer with simple preparation method, fast tumor formation, high tumor formation rate, and low cost. It is widely used in the study of invasion and metastasis mechanisms of esophageal squamous cell carcinoma. [0003] In the prior art, when preparing the metastasis model of esophageal squamous cell carcinoma, the method of injecting esophageal squamous cell carcinoma cells into the tail vein or intraperitoneal cavity is used to inoculate the esophageal squamous cell carcinoma cells into the mice, and the esophageal squamous cell carcinoma cells mostly produce lung, Liver and peritoneal metastasis, and lymph node metastasis is rarely found, and clinical metastasis is most...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/0271A01K2227/105A01K2267/0331
Inventor 范天黎刘红涛高盼惠怡然杨珍珍时壮壮杨振姚梦辉宋敏
Owner ZHENGZHOU UNIV
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