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Method for promoting CYP26 genetic expression of momordica grosvenori

A technology of gene expression and Luo Han Guo, applied in the field of plant biology, to achieve the effect of promoting concentrated expression, facilitating unified picking management, and increasing content

Inactive Publication Date: 2017-11-21
黄小华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on promoting the expression of the CYP26 gene in Luo Han Guo to increase the content of glucoside V in Luo Han Guo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0035] Cut off the short roots whose root length is less than 1 / 2 of the longest root length in the root system of Luo Han Guo tissue culture seedlings, and then cut 2 to 3 thick roots with a sterile knife, and then cover the group with medium. The root system of the cultured seedlings was treated with continuous variable temperature for 5 times, then magnetized, and finally the medium on the tissue cultured seedlings was removed wherein the concentration of methyl jasmonate added during the root system treatment process was 1.0 mg / L;

[0036] Wherein the medium is treated corn silk, and the corn silk needs to be washed and dried, soaked in a pre-soaking solution at a water temperature of 25°C for 2 hours, and the pre-soaking solution contains 1 mg / L nano-silver, 0.5 mg / L LBA, 1g / L sodium citrate, 3μg / L root powder, 0.15g / L glucomannan, with deionized water as solvent;

[0037] In the variable temperature treatment, the tissue-cultured seedlings are first stored at 2° C. for 1...

example 2

[0044] Cut off the short roots whose root length is less than 1 / 2 of the longest root length in the root system of Luo Han Guo tissue culture seedlings, and then cut 2 to 3 thick roots with a sterile knife, and then cover the group with medium. The root system of the cultured seedlings was treated with continuous variable temperature for 5 times, then magnetized, and finally the medium on the tissue cultured seedlings was removed wherein the concentration of methyl jasmonate added during the treatment was 2.0 mg / L;

[0045] Wherein the medium is treated corn silk, and the corn silk needs to be washed and dried, soaked in a pre-soaking solution at a water temperature of 25°C for 2 hours, and the pre-soaking solution contains 1 mg / L nano-silver, 0.5 mg / L LBA, 1g / L sodium citrate, 3μg / L root powder, 0.15g / L glucomannan, with deionized water as solvent;

[0046] In the variable temperature treatment, the tissue-cultured seedlings are first stored at 2° C. for 1 minute, and then st...

example 3

[0053] Cut off the short roots whose root length is less than 1 / 2 of the longest root length in the root system of Luo Han Guo tissue culture seedlings, and then cut 2 to 3 thick roots with a sterile knife, and then cover the group with medium. The root system of the cultured seedlings was treated with continuous variable temperature for 5 times, then magnetized, and finally the medium on the tissue cultured seedlings was removed wherein the concentration of methyl jasmonate added during the treatment was 1.5 mg / L;

[0054] Wherein the medium is treated corn silk, and the corn silk needs to be washed and dried, soaked in a pre-soaking solution at a water temperature of 25°C for 2 hours, and the pre-soaking solution contains 1 mg / L nano-silver, 0.5 mg / L LBA, 1g / L sodium citrate, 3μg / L root powder, 0.15g / L glucomannan, with deionized water as solvent;

[0055] In the variable temperature treatment, the tissue-cultured seedlings are first stored at 2° C. for 1 minute, and then st...

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PUM

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Abstract

The invention provides a method for promoting CYP26 genetic expression of momordica grosvenori. The method comprises the steps that root system treatment is conducted on tissue cultured seedlings of momordica grosvenori; special treatment is conducted on pollen after plants blossom, and 10 days after the plants blossom and are pollinated, a certain concentration of jasmonic acid methyl ester is added into inducing liquid which is continuously sprayed for 7 days. According to the method for promoting the CYP26 genetic expression of momordica grosvenori, the CYP26 genetic expression of momordica grosvenori can be promoted, and thus the accumulation of the content of mogrosideiv is promoted.

Description

technical field [0001] The invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for promoting the expression of CYP26 gene in Luo Han Guo. Background technique [0002] Luo Han Guo is a precious medicinal and sweet plant unique to my country. It is a perennial vine of the genus Siraitia in the family Cucurbitaceae. Its main component, glucoside V, is one of the strongest non-sugar sweeteners in the world, which is 300-400 times sweeter than sucrose. It is a low-calorie, pure natural sweetener and an ideal health product. It has anti-oxidation, immune regulation, anti-cancer, hypoglycemic and other effects. [0003] Sweet glycoside V cannot be chemically synthesized at present, and it is mainly obtained by extraction. Luo Han Guo has strict requirements on the habitat and is only suitable for growing in Guangxi, and it cannot be continuously cropped in cultivation. The content of glucoside V in the fruit is low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/04A01G7/06A01G31/00A01H1/02
CPCA01G7/04A01G7/06A01G31/00A01H1/02Y02P60/14
Inventor 黄小华韦荣昌
Owner 黄小华
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