Molecular breeding method of cold-tolerant rice

Abstract

The present invention provides a molecular breeding method of cold-tolerant rice. The molecular breeding method comprises: (1) carrying out hybridization by using a rice restoring line as a female parent and using a cold-tolerant rice line as a male parent to obtain F1 seed; (2) carrying out backcrossing on the rice restoring line as a recurrent parent and the hybridization F1 to obtain a first backcrossing generation group BC1 seed; (3) culturing the BC1 seed; and (4) screening the line containing the cold-tolerant gene from the BC1 lines, carrying out backcrossing on the obtained line and the rice restoring line, and carrying out selfing to obtain the rice line containing the cold-tolerant gene. According to the present invention, the excellent cold-tolerant rice resource is used, the new gene is excavated, the step-by-step molecular screening of the backcrossing group cold-tolerant material is performed, and the cold-tolerant material and the phenotype of the cold tolerance of the hybrid generation can be identified, such that the efficient transfer of the cold-tolerant gene can be achieved so as to create the new cold-resistant rice material.

Description

technical field [0001] The invention relates to the field of rice planting, in particular to a molecular breeding method for cold-tolerant rice. Background technique [0002] Low-temperature chilling damage to rice is common in many countries. There are more than 20 countries around the world that suffer from serious low-temperature chilling damage to rice. The area of ​​rice threatened by low-temperature chilling damage exceeds 15 million hectares 2 . Low temperature damage is more serious in Northeast China's rice fields. In southern China, rice is affected by low temperature in the early stage, which is easy to cause rotten seeds, rotten seedlings, and dead seedlings. In the later stage, it is affected by low temperature and normally heading and fruiting. In China, rice production is reduced by 30% every year due to low temperature damage. -5 billion kg. Because of the wide distribution and great harm of rice low-temperature chilling damage, it has become a hot spot in ...

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a kind of molecular breeding method of cold-tolerant rice, for solving the problems such as poor cold tolerance of rice in the prior art

Images