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Venenum bufonis extract with anticancer function and preparation method thereof

A technology of extract and toad venom, applied in the field of toad venom extract and its preparation, can solve the problems that affect the quality control of toad venom preparations, such as drug safety and unclear types and contents, so as to achieve drug safety, clear types and contents, and quality The effect of control

Inactive Publication Date: 2017-11-07
黄娇英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current marketed drugs mainly use the extract of toadseye as the active ingredient, but the types of active ingredients in the extract and the content of each ingredient are not clear, which seriously affects the quality control and drug safety of the toadseye preparation

Method used

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  • Venenum bufonis extract with anticancer function and preparation method thereof
  • Venenum bufonis extract with anticancer function and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of the Bufoni extract with anticancer effect

[0028] (1) Take 100 g of toad venom and add 1 L of ethanol aqueous solution with a volume fraction of 95%, soak at 25°C for 3 days, and filter to obtain the ethanol extract;

[0029] (2) Concentrate the ethanol extract until it has no alcohol smell, and then use a non-polar macroporous resin column to enrich the active ingredients of bufotoxins;

[0030] The specific method for enriching the active ingredients of bufatoxins in the non-polar macroporous resin column is as follows: after loading the ethanol extract on the non-polar macroporous resin column (D101 macroporous resin column), first elute with water to remove Miscellaneous, and then use 80% ethanol to elute the non-polar macroporous resin column; collect ethanol eluted parts, concentrate and dry to obtain the extract.

[0031] After testing, the active ingredients of bufotoxins in the extract prepared in this example accounted for 72% of the...

Embodiment 2

[0035] Example 2 The preparation of the toad venom extract with anticancer effect

[0036] (1) Take 100 g of toad venom and add 1 L of ethanol aqueous solution with a volume fraction of 95%, soak at 25°C for 3 days, and filter to obtain the ethanol extract;

[0037] (2) Concentrate the ethanol extract until it has no alcohol smell, and then use a non-polar macroporous resin column to enrich the active ingredients of bufotoxins;

[0038] The specific method for enriching the active ingredients of bufatoxins in the non-polar macroporous resin column is as follows: after loading the ethanol extract on the non-polar macroporous resin column (D101 macroporous resin column), first elute with water to remove Miscellaneous, and then use 60% ethanol to elute the non-polar macroporous resin column; collect the ethanol eluted parts, concentrate and dry to obtain the toad venom extract.

[0039] After testing, the active ingredients of bufotoxins in the extract prepared in this example a...

Embodiment 3

[0040] Example 3 The preparation of the extract of toad venom with anticancer effect

[0041] (1) Take 100 g of toad venom and add 1 L of ethanol aqueous solution with a volume fraction of 95%, soak at 25°C for 3 days, and filter to obtain the ethanol extract;

[0042] (2) Concentrate the ethanol extract until it has no alcohol smell, and then use a non-polar macroporous resin column to enrich the active ingredients of bufotoxins;

[0043] The specific method for enriching the active ingredients of bufatoxins in the non-polar macroporous resin column is as follows: after loading the ethanol extract on the non-polar macroporous resin column (D101 macroporous resin column), first elute with water to remove Miscellaneous, and then use 40% ethanol to elute the non-polar macroporous resin column; collect ethanol eluted parts, concentrate and dry to obtain the extract.

[0044] After testing, the active ingredients of bufotoxins in the extract prepared in this example accounted for...

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Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a toad venom extract with anticancer effect and a preparation method thereof. The toad venom extract contains active ingredients of bufa toxins, and the active ingredients of bufo toxins contain the following ingredients in parts by weight: 30-60 parts of bufa toxin essence; 30-60 parts of cinobufa toxin base; 10~25 parts; bufafetalin 25~50 parts; cinobubafalin 20~50 parts; bufofalin 25~50 parts; share. The toad venom extract of the present invention has remarkable anti-cancer effects, and in addition, the types and contents of the active ingredients of the extract are clear, which is beneficial to the quality control of the extract and the safety of medication.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a toad venom extract with anticancer effect and a preparation method thereof. Background technique [0002] Toad venom is the secretion of toad's post-auricular glands and epidermal glands, white milky liquid or light yellow serous, poisonous. Toad venom is extracted from the slurry, dried, colored, and can be used as medicine after being sealed and stored. It has many functions such as detoxification, analgesia, resuscitation, and antitumor, and is widely used. [0003] However, the currently marketed drugs mainly use toad venom extract as the active ingredient, but the types of active ingredients in the extract and the content of each component are not clear, which seriously affects the quality control and drug safety of the toad venom preparation. Therefore, it is of great significance to develop a toad venom extract with clear types and contents of act...

Claims

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Application Information

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IPC IPC(8): A61K35/65A61K31/585A61P35/00
CPCA61K35/65A61K31/585
Inventor 黄娇英
Owner 黄娇英
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