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Novel anti-neosporosis drug

A technology of Neospora caninum and drug, applied in the field of antiparasitic drugs

Inactive Publication Date: 2017-11-07
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no specific drug for the prevention and treatment of neosporosis. Although drugs such as pyrimethamine, sulfonamides, spiramycin, and clindamycin have certain curative effects, they have obvious shortcomings such as drug resistance and side effects. , people urgently need to find new effective drugs to block Neospora infection

Method used

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Examples

Experimental program
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Embodiment 1

[0021] The present invention takes SB203580 as an example to demonstrate its therapeutic effect on the invasion of cells by Neospora caninum, intracellular proliferation and mouse brain infection.

[0022] Pathways acting on MDBK cells:

[0023] After blocking host p38MAPK with SB203580, observe its effect on the invasion of host cells by N. caninum, and use SPSS software to analyze the data obtained statistically. Before infection (tachyzoites: cells = 10:1), the host cells were pre-incubated with SB203580 (20 µM) for 1 hour, and then washed with PBS to remove the inhibitor. The results showed that the percentage of infected cells decreased by 32.8% at 2 hours (P figure 1 shown).

[0024] Pathways acting on Neospora:

[0025] After blocking the parasite p38MAPK signaling pathway with SB203580, the effects on tachyzoite viability and actin secretion of N. caninum were observed, and the data obtained were statistically analyzed by SPSS software. Incubate with SB203580 (20 µM)...

Embodiment 2

[0032] The use of anti-Neospora caninum drugs

[0033] 1. Blocking effect of MAPK inhibitors on the invasion of Neospora caninum into cells

[0034] The present invention takes SB203580 as an example to observe its blocking effect on the invasion of MDBK cells by Neospora caninum.

[0035] MDBK cells were co-incubated with SB203580, and then inoculated with NC-1 tachyzoites (tachyzoites: cells = 10:1). Through cell invasion experiments, the blocking effect of inhibitors on Neospora caninum invasion cells was observed, and SPSS software was used to Statistical analysis was performed on the obtained data. The results showed that SB203580 had a concentration-dependent blocking effect on the invasion of Neospora caninum cells. When the concentration of SB203580 was 5 µM, 10uM and 20uM, the number of tachyzoites per 100 cells was reduced by 21.7 %, 39.8% and 63.5% (all P Figure 4 shown).

[0036] Blocking Effect of MAPK Inhibitor on Intracellular Proliferation of Neospora caninu...

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Abstract

The invention provides a novel anti-neosporosis drug, provides a new medical application of SB203580 and finds a chemical drug which can remarkably inhibit cell infection of neosporosis and infection of brain tissue of a mouse model and plays a blocking function by acting on host cells and parasites simultaneously. A novel candidate drug and a novel candidate drug action target p38 MAPK are provided for treatment of neosporosis, and a foundation is laid for future drugs for intervening the neosporosis.

Description

technical field [0001] The invention provides a new anti-Neospora canis drug, and provides a new application of SB203580 for the treatment of neosporosis, belonging to the technical field of anti-parasitic drugs. Background technique [0002] Neosporiasis is caused by Neospora canis ( Neosporacaninum ) is a fatal protozoan disease caused by parasitizing in the cells of the vertebrate host, which is a newly discovered protozoan disease. Like most members of the Apicomplexa, Neospora parasitizes exclusively in the nucleated cells of the host, and its host range is wide, including most mammals. It mainly leads to the abortion of pregnant animals, the death of young animals and the disorder of motor nervous system. According to statistics, 12%-42% of dairy cow abortions in the world are caused by Neospora infection, so Neosporosis is considered to be the main cause of dairy cow abortion worldwide, and it is an important primary pathogen for cattle. The animal husbandry, espec...

Claims

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Application Information

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IPC IPC(8): A61K31/4439A61P33/02
CPCA61K31/4439
Inventor 李建华靳小霞张西臣宫鹏涛祝涛杨正涛杨举李赫
Owner JILIN UNIV
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