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Application of BK channel opener in establishing vascular smooth muscle cell calcification model

A technology of vascular smooth muscle and smooth muscle, applied in the field of application

Inactive Publication Date: 2017-10-31
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] NS1619 is a kind of BK channel opener, and there is no report about the effect of NS1619 on inhibiting vascular smooth muscle cell calcification

Method used

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  • Application of BK channel opener in establishing vascular smooth muscle cell calcification model
  • Application of BK channel opener in establishing vascular smooth muscle cell calcification model
  • Application of BK channel opener in establishing vascular smooth muscle cell calcification model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and identification of primary vascular smooth muscle cells

[0028] 1) Isolation of primary vascular smooth muscle cells

[0029] Two to three male rats were killed by dislocation of the cervical vertebrae, soaked in 75% ethanol for 3 minutes, fixed and then cut open the chest and abdomen skin, muscles and sternum to expose the chest cavity. The aorta was put into a cell culture dish filled with PBS buffer, quickly transferred to the ultra-clean workbench of the cell culture room, and transferred to another cell culture dish filled with PBS buffer after routine treatment. After trimming the blood vessels, Transfer to a cell culture dish containing 20% ​​fetal bovine serum and DMEM mixture, cut open the blood vessel lumen with ophthalmic straight scissors, with the intima facing up, gently wipe the intima layer with ophthalmic curved forceps or a scalpel blade to remove endothelial cells, Finally, transfer it to another cell culture dish containing 20% ​​fe...

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PUM

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Abstract

The invention belongs to the field of bio-pharmaceuticals, relates to a novel application of a novel inhibitor large-conductance calcium-activated potassium channel (also called a BK channel) opener NS1619 influencing a process of establishing a vascular smooth muscle cell calcification model, specifically relates to an application of the NS1619 used for establishing the vascular smooth muscle cell calcification model, and in particular relates to a novel application of the NS1619 used for preparing drugs for inhibiting vascular smooth muscle cell calcification. Cell experiment results prove that the NS1619 has effects of inhibiting vascular smooth muscle cell calcification, including an effect of inhibiting Ca<2+> deposition in the calcification process, an effect of inhibiting molecular expression of a calcification marker molecule and an effect of inhibiting formation of calcium nodules. The NS1619 can be further used for preparing drugs for treating diseases related to vascular sclerosis and smooth muscle contraction or relaxation dysfunction, including treatment of atherosclerosis, asthma, cerebral ischemic injury, uroschesis and other smooth muscle dysfunction related diseases.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to a new application of a large conductance calcium-activated potassium channel (also known as BK channel) opener NS1619, which affects the process of establishing a vascular smooth muscle cell calcification model, and specifically relates to the use of NS1619 in establishing blood vessels The application in smooth muscle cell calcification model, especially the new application of NS1619 in the preparation of inhibiting vascular smooth muscle cell calcification. Background technique [0002] CKD-MBD (chronic kidney disease-mineral and bone disorder) refers to the systemic disorder of mineral and bone metabolism caused by chronic kidney disease. The prior art discloses that vascular calcification is a common complication of CKD. Patients with various stages of CKD have varying degrees of calcium and phosphorus metabolism disorders. The resulting vascular calcification is an independen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4184A61P9/10A61P11/06A61P9/14A61P7/10
CPCA61K31/4184
Inventor 张雪梅彭文田露露陶杰王利曹爱丽
Owner FUDAN UNIV
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