MALDI-TOF-MS-based dead slaughtered meat identification method and system

A MALDI-TOF-MS, live slaughtering technology, used in character and pattern recognition, material inspection products, instruments, etc., can solve the problems of low accuracy and poor reproducibility of detection methods, and achieve high stability and good reproducibility. Sex, reduce the effect of self-factor interference

Active Publication Date: 2017-10-27
融智生物科技(青岛)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, in order to solve the problems of poor reproducibility and low accuracy of existing detection methods and establish a scientific, efficient and accurate identification method and system, it is necessary to provide a method and system for identifying dead meat based on MALDI-TOF-MS

Method used

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  • MALDI-TOF-MS-based dead slaughtered meat identification method and system
  • MALDI-TOF-MS-based dead slaughtered meat identification method and system
  • MALDI-TOF-MS-based dead slaughtered meat identification method and system

Examples

Experimental program
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Effect test

example 1

[0117] (1) Prepare meat infusion.

[0118] 20 batches of live slaughtered pork samples were obtained from a designated slaughtering and processing enterprise, and 6 samples in each batch were tested in parallel, totaling 120 samples. After removing fat and connective tissue from each sample (choose tenderloin, abdominal wall, brachialis, brachialis biceps, brachialis medius, semitendinosus, etc.), weigh 25g into a sterile homogeneous bag with a strainer Add 250mL of double distilled water, beat on a beating homogenizer for 2 minutes (beating rate is 4 times / second), remove the homogenizing bag, filter about 50mL into a centrifuge tube, and let it stand at 4°C for 2 hours. Use a straw to degrease the surface to obtain a meat dip.

[0119] (2) MALDI-TOF measurement.

[0120] The meat extract was mixed with sinapinic acid matrix solution (SA) in equal volumes, and sampled, 2 μL for each target. After drying the crystals, put them into a MALDI-TOF instrument, scan and measure, ...

example 2

[0125] (1) Prepare meat infusion.

[0126] 15 batches of diseased and dead pork were obtained from 2 pig farms, and 6 parallel tests were performed on each batch of samples, and the number of samples was 90. After removing fat and connective tissue from each sample (choose tenderloin, abdominal wall, brachialis, brachialis biceps, brachialis medius, semitendinosus, etc.), weigh 10g into a sterile homogeneous bag with a filter Add 100mL of double-distilled water, beat on a beating homogenizer for 3 minutes (beating rate is 4 times / second), remove the homogenizing bag, filter about 50mL into a centrifuge tube, and let it stand at 4°C for 2 hours. Use a straw to degrease the surface to obtain a meat dip.

[0127] (2) MALDI-TOF measurement.

[0128] The meat extract was mixed with sinapinic acid matrix solution (SA) in equal volumes, and sampled, 1 μL for each target. After drying the crystals, put them into a MALDI-TOF instrument, scan and measure, and obtain a mass spectrum. ...

example 3

[0133] (1) Prepare meat infusion.

[0134] A total of 30 batches of pork samples were obtained, of which 15 batches were diseased and dead pork obtained from 4 pig farms, and 15 batches were live slaughtered pigs obtained from a designated slaughterhouse in Hebei Province. Each batch of samples was tested in parallel 6, the number of samples is 180. After removing fat and connective tissue of each sample (choose tenderloin, abdominal wall, brachialis, brachialis biceps, brachialis medius, semitendinosus, etc.), weigh 20g into a sterile homogeneous bag with filter Add 200mL of double-distilled water, beat on a beating homogenizer for 2 minutes (beating rate is 4 times / second), remove the homogenizing bag, filter about 50mL into a centrifuge tube, and let it stand at 4°C for 2 hours. Use a straw to degrease the surface to obtain a meat dip.

[0135] (2) MALDI-TOF measurement.

[0136] The meat extract was mixed with sinapinic acid matrix solution (SA) in equal volumes, and sa...

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Abstract

The invention discloses an MALDI-TOF-MS-based dead slaughtered meat identification method. The method comprises the following steps: preparing a sample set, wherein the sample set comprises a training sample set, a testing sample set and a to-be-measured sample set; measuring the sample sets by using the MALDI-TOF-MS to obtain a corresponding mass spectrum; establishing a neural work classifier based on the mass spectrum of the training sample set and the testing sample set; classifying the samples in the to-be-measured sample set by applying the neural network classifier to obtain the identification result. The invention also discloses an MALDI-TOF-MS-based dead slaughtered meat identification system. The metabolomic and proteomic difference of dead slaughtered meat and living slaughtered meat is found in the molecular level by virtue of the mass spectrometer, interference of environmental factors and sample factors is reduced maximally through training study and testing of the neutral network classifier, the detection process has high stability, sensitivity and accuracy, the detection result has high reproducibility, and the MALDI-TOF-MS based dead slaughtered meat identification method and the MALDI-TOF-MS based dead slaughtered meat identification system are a scientific, accurate and efficient dead slaughtered meat identification method as well as a scientific, accurate and efficient dead slaughtered meat identification system.

Description

technical field [0001] The invention relates to a meat quality identification method and system. More specifically, it relates to a method and system for identifying dead meat based on MALDI-TOF-MS. Background technique [0002] With the improvement of modern living standards, people's demand for high-quality meat is becoming stronger and stronger. Compared with the live slaughtered meat that is purchased and slaughtered live animals and enters the market after quarantine, dead slaughtered meat is a kind of meat that privately purchases and slaughters sick and dead animals and escapes quarantine and enters the market. It has biological hazards, toxic and harmful substances, and The hazards of drug residues pose a great threat to people's quality of life. Therefore, more and more attention has been paid to the inspection and identification of dead slaughtered meat in the market. [0003] Taking dead pork as an example, there are mainly two methods for testing dead pork on ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N27/64G06K9/62
CPCG01N27/64G01N33/6851G06F18/214G06F18/241
Inventor 佟雪梅宋合兴刘元杰李运涛周晓光
Owner 融智生物科技(青岛)有限公司
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