Application of compound epothilone B to preparing medicine for healing cornea neurotrauma

A nerve trauma and compound technology, applied in the field of biomedicine, can solve problems such as accelerating corneal nerve trauma repair and functional recovery, achieve important development and application prospects, promote repair and reconstruction, and reduce eye symptoms.

Active Publication Date: 2017-10-27
广州昇藤教育科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Application of compound epothilone B to preparing medicine for healing cornea neurotrauma
  • Application of compound epothilone B to preparing medicine for healing cornea neurotrauma
  • Application of compound epothilone B to preparing medicine for healing cornea neurotrauma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, EpoB accelerates mouse corneal nerve trauma repair

[0025] 1. Experimental animals

[0026] 8-week-old C57 / BL6 female mice, weighing 15-20 g, were purchased from the Experimental Animal Center of Guangdong Province. Before the experiment, the eyes of the 24 mice were checked to ensure that the experimental mice had no eye diseases, corneal defects and conjunctival damage.

[0027] 2. Experimental Drugs

[0028] Dissolve EpoB in DMSO (dimethyl sulfoxide) to prepare a solution with a concentration of 5 mg / mL for use. The EpoB stock solution was diluted with normal saline at a ratio of 1:200, the mice were intraperitoneally injected with 0.1 mg / mL EpoB, and the mice in the control group were intraperitoneally injected with the same amount of blank solvent.

[0029] 3. Experimental method

[0030] Twenty normal mice without eye diseases were screened and randomly divided into trauma control group and EpoB administration group. After the mice in each grou...

Embodiment 2

[0033] Example 2, EpoB accelerates functional recovery of injured corneal nerves in rats

[0034] 1. Experimental animals

[0035] Thirty healthy 8-week-old male SD rats, weighing about 250±20g, were purchased from Guangdong Experimental Animal Center. The eyes of the animals were examined within 24 hours before the experiment, and animals without symptoms of eye irritation, corneal defects, or conjunctival damage were used for the experiment.

[0036] 2. Experimental Drugs

[0037] SR8278 (purchased from Sigma, USA) was dissolved in an appropriate amount of DMSO, and soybean oil for injection was added to prepare SR8278 oily eye drops with a drug content of 0.2%.

[0038] 3. Establishment of rat corneal nerve injury animal model

[0039] Rats were anesthetized with Sumianxin (purchased from Jilin Province Huamu Animal Health Products Co., Ltd.), and a corneal trephine with a diameter of 3 mm and a depth of 0.5 mm was drilled in the center of the cornea under a microscope. ...

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Abstract

The invention discloses application of a compound epothilone B to preparing a medicine for healing cornea neurotrauma, and belongs to the field of biological medicines. The pharmacodynamic experiment proves that a rat suffering from cornea neurotrauma is taken as an experimental subject and the area of a cornea nerve is obviously increased by EpoB and is close to the normal level. The compound EpoB stabilizes the tubulin binding, so that the tubulin polymerization is promoted, the depolymerization of a microtube is inhibited, the concentration of the tubulin is increased, the repairing and the reconstruction of the neurotrauma are promoted, and the normal structure and functions of the cornea are maintained. The EpoB has better treatment effects on the function injuries and the cornea nerve lesion caused by refractive surgery, keratoplasty, herpes simplex keratitis, xerophthalmia, diabetes and long-term use of eyedrops, and alleviates the eye symptoms of a patient; and the medicine is low in toxicity and high in stability, and has an important development and application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of a compound Epothilone B (Epothilone B, EpoB) in the preparation of medicaments for repairing corneal nerve trauma. Background technique [0002] Since Sohlemm discovered the corneal nerve in the limbus in 1830, the study of the corneal nerve has made great progress. Corneal tissue has no blood vessels but is rich in nerve fibers. It is one of the most nerve-dense tissues in the human body and plays an important role in providing nutrient metabolism and maintaining the normal structure and function of the cornea. Corneal sensation is the most prominent feature of corneal nerve, which has a keen sensory function. Corneal nerves are peripheral nerves with incomplete regenerative capacity. Although corneal nerves can slowly regenerate after trauma, it is difficult to restore normal nerve density and function. [0003] Corneal nerve abnormalities can be caus...

Claims

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Application Information

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IPC IPC(8): A61K31/427A61P25/02A61P27/02
CPCA61K31/427
Inventor 李志杰董栋王寒晴肖程聚薛芸霞
Owner 广州昇藤教育科技有限公司
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