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Mediums for tissue culture of Meipian tree and culturing method

A technology of tissue culture and culture medium, applied in the field of plant tissue culture, to achieve the effect of increasing the bud induction rate, shortening the reproduction cycle, and high-efficiency tissue culture and rapid propagation system

Active Publication Date: 2017-10-24
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the medium has a higher rate of bud induction to Prunus quinquefolius, there is no report on the medium for the bud proliferation and rooting stages of Prunus quinquefolius

Method used

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  • Mediums for tissue culture of Meipian tree and culturing method
  • Mediums for tissue culture of Meipian tree and culturing method
  • Mediums for tissue culture of Meipian tree and culturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The tissue culture of embodiment 1 plum slice tree

[0043] (1) Collect healthy branches of the annual plum tree with buds near the base from April to May as explants, avoid collecting in rainy days, remove the leaves and keep the petioles, and then soak in detergent to remove surface impurities.

[0044] (2) Cut the explants into 2-3 cm stem segments with 1-2 axillary buds in the ultra-clean workbench, first soak them in 75% alcohol for 1 min, and then rinse them with sterile water 6 times, each time Soak for 1 min; then sterilize by soaking in 0.1% mercuric chloride solution for 10 min, and finally rinse with sterile water for 6 times, soaking for 1 min each time.

[0045] (3) Inoculate the treated explants into the subpackaged primary medium, wherein the primary medium is: DCR medium+2mg / L 6-BA+0.05mg / L NAA+10g / L agar and 30g / L sucrose, the pH value is 5.8. Place in a culture room with 16h / d light, maintain a temperature of 25±1°C, a light intensity of 3000lx, and ...

Embodiment 2

[0049] The tissue culture of embodiment 2 plum slice tree

[0050] (1) Collect healthy branches of the annual plum tree with buds near the base from April to May as explants, avoid collecting in rainy days, remove the leaves and keep the petioles, and then soak in detergent to remove surface impurities.

[0051] (2) Cut the explants into stem segments of about 2-3 cm with 1-2 axillary buds in the ultra-clean workbench (such as figure 1 shown), soak in 75% alcohol for 2 minutes, then rinse with sterile water for 6 times, soaking for 1 minute each time; Soak for 1min.

[0052] (3) Inoculate the treated explants into the subpackaged primary medium, wherein the primary medium is: DCR medium+2mg / L 6-BA+0.1mg / L NAA+10g / L agar+ 30g / L sucrose, the pH value is 5.9. Place in a culture room with 16h / d light, maintain a temperature of 25±1°C, a light intensity of 3000lx, and a relative humidity of 34%. After 30 days of culture, the explants grew axillary buds, recorded the budding sit...

Embodiment 3

[0056] The tissue culture of embodiment 3 plum slice tree

[0057] The primary medium is: DCR medium+3mg / L 6-BA+0.05mg / L NAA+10g / L agar and 30g / L sucrose, the pH value is 5.8;

[0058] The subculture medium is: DCR medium + 5mg / L 6-BA + 0.1mg / L NAA + 10g / L agar + 30g / L sucrose, the pH value is 5.8;

[0059] The rooting medium is: DCR medium+1.5mg / L IBA+10g / L agar+10g / L sucrose, the pH value is 5.8.

[0060] Other operations are the same as in Example 1.

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Abstract

The invention especially relates to mediums for tissue culture of a Meipian tree and a culturing method, belonging to the technical field of plant tissue culture. According to the invention, the twig of the Meipian tree is used as an explant; a specific primary medium, a specific subculture medium and a specific rooting medium are selected; plant tissue culture techniques are utilized; and a novel approach is employed for rapid propagation in tissue culture of the Meipian tree. The mediums and the culturing method in the invention substantially improve the bud induction rate, propagation coefficient and rooting rate of the Meipian tree, shorten a propagation period and provide a more effective approach for industrial production of the Meipian tree.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium and a culture method for the tissue culture of Prunus chinensis. Background technique [0002] Natural d-borneol has a very wide range of applications in the field of medicine and health care, and the plum tree in the Lauraceae plant is an important source of d-borneol. Although the traditional cutting and rooting method can also achieve the purpose of reproduction, it has the disadvantages of easy aging of plants and few years of flowering and fruiting. Plant tissue culture refers to the use of sterile techniques to cultivate isolated parts of plants, such as root tips, shoot tips, leaves, embryos, seeds, fruits, and various tissues and cells, protoplasts, etc. The theoretical basis of plant tissue culture is the totipotency of plant cells, that is, each cell of a plant contains all the genetic information of the species, so it has the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 马笑宇傅明辉
Owner GUANGDONG UNIV OF TECH
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