A kind of immune p16ink4a antigen preservation solution for liquid-based thin layer cells and preparation method thereof

Abstract

The invention belongs to the technical field of clinical medicine pathology and particularly relates to an immune p16<INK4a> antigen preserving liquid used for thin prep liquid-based cytology test and a preparation method of same. The antigen preserving liquid includes: 60-100 mmol / L of NaCl, 2-10 mmol / L of KCl, 1-5 mmol / L of EDTA disodium, 30-50 mmol / L of urea, 1-5 mmol / L of sodium citrate, 5-20 mmol / L of sucrose, 4-10 mmol / L of PEG-400, 2-3% of paraformaldehyde, 2-10 mmol / L of glycerol and 10-20 mmol / L of dithiothreitol. The preserving liquid is not only suitable for traditional slide preparation and staining of liquid-based cells, but also is suitable for cervical liquid-based cell p16<INK4a> protein immuno-labeling and staining, thus avoiding defects in conventional non-specific Papanicolaou staining.

Description

technical field [0001] The invention belongs to the technical field of clinical medical pathology, in particular to a p16 immune system for liquid-based thin layer cells INK4a Antigen preservation solution and preparation method thereof. Background technique [0002] Cervical cancer is one of the most common malignant tumors of the female reproductive system in gynecology. Its incidence is second only to breast cancer and ranks second among female malignant tumors. It seriously threatens women's health and quality of life. China is a country with a high incidence of cervical cancer, accounting for about 1 / 3 of the world's incidence and mortality. Cervical cancer is currently the only cancer whose etiology is clear and can be completely cured through effective early diagnosis and treatment. [0003] The current early diagnosis of cervical cancer and precancerous lesions is divided into "three steps". The first step is vaginal exfoliation cytology examination to screen out ...

AI Technical Summary

Problems solved by technology

[0013] p16 INK4a For the application in cytology, a suitable cell preservation solution is first required. The traditional liquid-based cell preservation solution has the functions of lysing red blood cells, diluting mucus, and fixing cells, which can meet the requirements of liquid-based thin-layer preparation technology. However, the The chemical reagents used are prone to cause p16 INK4a Blocking of epitopes, p16 INK4a Antigen cannot play a very good protective role, which can easily lead to false negative staining results

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