A kind of mlh fusion antimicrobial peptide and its preparation method and application

Abstract

The invention discloses an MLH fusion antibacterial peptide, a preparation method and application thereof. Housefly antibacterial peptide, LL-37 and helicobacter pylori peptide are adopted as the parent peptide, a bioinformatics method is utilized for analysis to obtain an amino acid sequence of MLH, and a gene fragment of the hybrid gene MLH is encoded according to the E.coli codon preference. SOE-PCR technology is employed to synthesize a required target gene, the target gene is connected to the expression vector pET-32a(+), and the obtained recombinant expression vector is transferred into an escherichia coli expression strain to construct the recombinant genetic engineering strain pET-32a-MLH / BL21(DE3), IPTG induced protein fusion expression is carried out, and finally bacteriostatic activity identification is conducted on the obtained fusion expressed hybrid peptide, and the result indicates that the fusion expressed hybrid peptide has significant bacteriostatic effect on staphylococcus aureus and bacillus subtilis.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a MLH fusion antibacterial peptide and its preparation method and application. Background technique [0002] In recent years, the abuse of antibiotics has become increasingly serious, and more and more bacteria have developed into strains resistant to traditional antibiotics. Antimicrobial peptides have become ideal substitutes for antibiotics due to their stable physical and chemical properties, broad-spectrum antibacterial properties, and resistance to target strains, and have received extensive attention. Antimicrobial peptides are a class of small molecule polypeptide substances that widely exist in organisms in nature and are an important part of the body's innate immunity. It has a different target and mechanism of action from antibiotics, and it also has a good antibacterial effect on antibiotic-resistant bacteria, and has broad-spectrum antibacter...

AI Technical Summary

Problems solved by technology

Due to the low content of antimicrobial peptides in organisms, direct extraction is difficult and expensive, and it is difficult to achieve mass production; chemical synthesis methods are also rarely used in large-scale production due to high costs

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