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Preparation method of Chinese wisteria seed aphis-killing active ingredients

A technology for insecticidal activity and seeds, which is applied in the field of preparation of aphidicidal active components of wisteria seeds, can solve the problems of technical disclosure and no aphidicidal activity of no aphidicidal active components, and achieve good control of aphids Hazardous, high contact activity, not easy to produce resistance effect

Inactive Publication Date: 2017-10-20
INT CENT FOR BAMBOO & RATTAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no technical publication on the aphidicidal activity of wisteria seed extract, and there is no technical publication on the preparation method of the aphidicidal active component of wisteria seed extract

Method used

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  • Preparation method of Chinese wisteria seed aphis-killing active ingredients
  • Preparation method of Chinese wisteria seed aphis-killing active ingredients
  • Preparation method of Chinese wisteria seed aphis-killing active ingredients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Test insects

[0017] Cotton aphid (Aphis gossypii Glover) was collected in a cotton field in the suburb of Langfang City, Hebei Province in August 2013, and the cotton seedlings cultivated with Holland nutrient solution were propagated for several generations indoors.

[0018] Preparation of test solution

[0019] Wisteria seed extract will be obtained, and each fraction separated by silica gel column chromatography (including petroleum ether, petroleum ether: ethyl acetate (7: 3, v / v), ethyl acetate, ethyl acetate: methanol (6: 4 , v / v), ethyl acetate:methanol (2:8, v / v), methanol and other fractions) after accurately weighing an appropriate amount, dissolve it with acetone, prepare solutions with different mass concentrations, and store them for testing.

[0020] Bioassay

[0021] 1. Contact activity test - drop method, the contact activity of active fractions of wisteria seed extracts on cotton aphids was carried out by drop method, according to the agricultural i...

Embodiment 2

[0041] with supercritical CO 2 After fluid extraction, the oil phase is collected to obtain the crude extract of wisteria seeds. Then adopt the same bioassay method as in Example 1 and further silica gel column chromatographic separation to obtain substantially the same effect as in Example 1.

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Abstract

The invention provides a preparation method of Chinese wisteria seed aphis-killing active ingredients, and belongs to the technical field of an insecticide. According to the method, Chinese wisteria seeds are dried and crushed; then, after the leaching and reduced pressure concentration, Chinese wisteria seed coarse extracts are obtained; through a silica gel chromatography column, different solvents are used for sequentially eluting a chromatographic column, wherein particularly when an eluting agent is ethyl acetate and methanol according to a ratio of 2 / 8 (v / v), the obtained eluting cut fraction is aphis-killing active ingredients. The Chinese wisteria seed aphis-killing active ingredients provided by the invention have high contact killing activity on aphis, and can be used for preventing and controlling pests of vegetable and cotton aphis. In addition, the aphis-killing active ingredients are from the plant Chinese wisteria seeds, so that the characteristics of safety, high efficiency, resistance generating difficulty on pests and the like are achieved. The active ingredients can be used as the active ingredients of the plant aphis-killing agents to be singly used or to be used by being compounded with other plant source pesticides.

Description

technical field [0001] The invention relates to a preparation method of an aphidicidal active component of wisteria seeds, belonging to the technical field of insecticides. Background technique [0002] Cotton aphid (Aphis gossypii Glover) belongs to Hemiptera Hemiptera, Aphididae Aphididae, is an important agricultural pest, and has harmful effects on cotton and other crops. It not only sucks plant juice and destroys plant growth, but also spreads plant virus diseases, causing serious losses to agricultural production. In production, chemical pesticides are commonly used to control cotton aphids, but it is easy to cause problems such as drug resistance and pesticide residues of cotton aphids. Therefore, the use of botanical pesticides to control cotton aphids is the main direction for the future development of new pesticide research and development. [0003] Wisteria sinensis Sweet is a vine of the genus Wisteria, widely distributed in my country and rich in resources. Wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/20A01P7/04
CPCA01N65/00A01N65/20
Inventor 王进岳永德汤锋曹先爽宋丽
Owner INT CENT FOR BAMBOO & RATTAN
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