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Tissue-culture rapid-propagation method for Cardiospermum halicacabum

A technology of tissue culture and bollium, which is applied in the field of tissue culture and rapid propagation of boll, can solve the problems of low germination rate and seedling rate, hinder the rapid and large-scale propagation of boll, and prevent other diseases from occurring , Inhibit the interconversion of dopachrome, enhance the effect of disease resistance and stress resistance

Active Publication Date: 2017-10-13
黄小燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bombardier is usually an annual plant, which is propagated by seed sowing, but the germination rate and seedling rate of the traditional sowing propagation method are not high, which hinders the rapid and large-scale propagation of Bombardier, so there is an urgent need for a plant that can Method for Realizing the Rapid Propagation of Boll's Boll Seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A tissue culture rapid propagation method of bells, comprising the following steps:

[0033] S1. Explant treatment: take the seeds of Bombinus chinensis as explants, first soak them with the extract of kaleba japonica for 10 minutes, then rinse them with distilled water for 5 minutes, then soak them with 2% sodium hypochlorite solution for 10 minutes, and then use sterile water Rinse for 15 minutes, and finally remove surface moisture with sterilized filter paper to obtain the treated explants;

[0034] Wherein, the extracting solution of kaleba is made of the following components in parts by weight: 50 parts of fresh kale, 10 parts of fresh elderberry leaves, 8 parts of fresh red orchid, 10 parts of ethanol and 300 parts of sterile water; A few parts of fresh collard grass, fresh elder leaves, and fresh red orchids were smashed, and then the above-mentioned parts by weight of ethanol and sterile water were added, and the ultrasound was performed at a frequency of 30KHz...

Embodiment 2

[0046] A tissue culture rapid propagation method of bells, comprising the following steps:

[0047] S1, explant treatment: take the seeds of Bollia chinensis as explants, first soak for 15 minutes with the leaching solution of kaleba, then rinse with distilled water for 10 minutes, then soak for 15 minutes with a mass fraction of 5% sodium hypochlorite solution, and then use sterile water Rinse for 20 minutes, and finally remove surface moisture with sterile filter paper to obtain the treated explants;

[0048] Wherein, the extracting solution of kaleba is made of the following components in parts by weight: 60 parts of fresh kale, 15 parts of fresh elderberry leaves, 10 parts of fresh red orchid, 15 parts of ethanol and 500 parts of sterile water; A few parts of fresh collard grass, fresh elder leaves, and fresh red orchids were smashed, and then the above-mentioned parts by weight of ethanol and sterile water were added, and the ultrasonic temperature was controlled at 35°C ...

Embodiment 3

[0060] A tissue culture rapid propagation method of bells, comprising the following steps:

[0061] S1, explant treatment: take the seeds of Bombinus chinensis as explants, first soak for 12 minutes with the leaching solution of kale, then rinse with distilled water for 8 minutes, then soak for 12 minutes with a mass fraction of 3.5% sodium hypochlorite solution, and then use sterile water Rinse for 17 minutes, and finally remove surface moisture with sterile filter paper to obtain the treated explants;

[0062] Wherein, the extracting solution of kaleba is made of the following components in parts by weight: 55 parts of fresh kale, 12 parts of fresh elderberry leaves, 9 parts of fresh red orchid, 12 parts of ethanol and 400 parts of sterile water; A few parts of fresh collard grass, fresh elder leaves, and fresh red orchids were smashed, and then the above-mentioned parts by weight of ethanol and sterile water were added, and ultrasonic waves were used for 22 minutes at a fre...

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Abstract

The invention discloses a tissue-culture rapid-propagation method for Cardiospermum halicacabum. The method comprises the following steps: explant treatment: taking Cardiospermum halicacabum seeds as explants, and soaking the explants with an Alchemilla vulgaris lixiviate for 10 to 15 minutes; explant culture: inoculating an MS explant culture medium containing L-Se-methylselenocysteine, vitamin C and taurine with B the treated explants; propagation culture; seedling strengthening culture: putting test-tube plantlet cluster buds obtained through the propagation culture in an MS seedling strengthening culture medium, wherein the MS seedling strengthening culture medium contains L-Se-methylselenocysteine, activated charcoal and kanamycin and is further added with tourmaline powder and far-infrared ceramic powder; rooting culture; and seedling hardening and transplanting. According to the method, the browning rate and vitrification rate of tissue culture seedlings can be lowered, the transplanting survival rate is increased, meanwhile, the percentage of establishment of the Cardiospermum halicacabum is increased, the rapid propagation of the Cardiospermum halicacabum can be achieved, and thus, the large-scale propagation of the Cardiospermum halicacabum is achieved.

Description

technical field [0001] The invention relates to the technical field of the breeding of the bougainvillea, in particular to a rapid propagation method for the tissue culture of the bougainvillea. Background technique [0002] Fallen bell is also known as heart bean vine or white heart seed, and it is also known as false bitter gourd, sailboat kudzu, neem with vine, and lantern grass. It is a perennial woody vine of Sapindaceae. Cooling blood, removing blood stasis, detoxifying effect, can cure pneumonia, jaundice, diabetes, gonorrhea, furuncle, rheumatism, bruises, snake bites, etc. It is also a common ornamental plant. The bell flowers are white, the fruit is enlarged and nearly spherical, the seeds are black, and there are heart-shaped white spots. Bombardier is usually an annual plant, which is propagated by seed sowing, but the germination rate and seedling rate of the traditional sowing propagation method are not high, which hinders the rapid and large-scale propagation...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G7/04A01N65/12A01N59/00A01P1/00
CPCA01G7/04A01H4/001A01H4/008A01N59/00A01N65/00A01N65/12A01N2300/00Y02P60/14
Inventor 黄小燕韦荣昌
Owner 黄小燕
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