A surface acoustic wave-based cell adhesion measuring instrument and measuring method

Abstract

The invention discloses a cell adhesion force measuring instrument and measuring method based on acoustic surface waves. The cell adhesion force measuring instrument comprises a lithium niobate crystal plate, a silver interdigitatal electrode annular array, a PDMS (polydimethylsiloxane) micro-channel and a micro-vision servo system, the micro-vision servo system comprises an upper computer system based on a PXI (peripheral component interconnect) bus, a multi-path signal generating system and a micro-vision monitoring system, the silver interdigitatal electrode annular array is arranged on the upper periphery of the lithium niobate crystal plate, the lithium niobate crystal plate and the silver interdigitatal electrode annular array form an interdigital transducer, and the silver interdigitatal electrode annular array is connected with the multi-path signal generating system. According to the measuring instrument, an expected sound field is synthesized in a PDMS micro-channel cavity by the aid of the interdigital transducer and the micro-vision servo system, cells are operated by the aid of radiation force of the acoustic surface waves, cell adhesion force is measured, and the measuring instrument has the advantages of simplicity and easiness in operation, fewer interference factors and the like.

Description

technical field [0001] The invention relates to a cell adhesion measuring device, in particular to a surface acoustic wave-based cell adhesion measuring instrument and a measuring method. Background technique [0002] With the continuous development of science and technology, many research groups in the field of biology and engineering have conducted a lot of research on biomechanics, and found that changes in cell mechanical properties may be used as a means of detecting and screening a small number of abnormal cells and early diagnosis of fatal diseases in accordance with. Therefore, the study of cell biomechanics can bring great value to the breakthrough and application of basic sciences. Cell adhesion is one of the important mechanical properties of cells, which refers to the mutual adhesion and interaction between cells, cells and other materials, surfaces, substrates or matrices. For example, the mechanical and chemical coupling between new cells and old cells; the s...

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Problems solved by technology

This method is complicated to make cell probes, and the cells need to be fixed with the probes, which may affect the mechanical properties of the cells themselves, and there are many interference factors. Measuring the adhesion force through the deformation of the probe cantilever beam requires extremely high sensitivity of the equipment, and the measurement accuracy is difficult. ensure

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