A surface acoustic wave-based cell adhesion measuring instrument and measuring method

Abstract

The invention discloses a cell adhesion measuring instrument and a measuring method based on surface acoustic waves. It includes lithium niobate wafer, silver interdigital electrode annular array, PDMS micro-channel and micro visual servo system. The micro visual servo system includes a host computer system based on PXI bus, a multi-channel signal generation system and a micro visual monitoring system. , a lithium niobate wafer is surrounded by a silver interdigital electrode annular array, the lithium niobate wafer and the silver interdigital electrode annular array form an interdigital transducer, the silver interdigital electrode annular array and a multi-channel signal generation system connected. The present invention uses an interdigital transducer and a microscopic visual servo system to synthesize the expected sound field in the PDMS microfluidic cavity, uses surface acoustic wave radiation force to manipulate cells and measures cell adhesion, and has the advantages of simple operation and low interference factors. Advantages of waiting less.

Description

technical field [0001] The invention relates to a cell adhesion measuring device, in particular to a surface acoustic wave-based cell adhesion measuring instrument and a measuring method. Background technique [0002] With the continuous development of science and technology, many research groups in the field of biology and engineering have conducted a lot of research on biomechanics, and found that changes in cell mechanical properties may be used as a means of detecting and screening a small number of abnormal cells and early diagnosis of fatal diseases in accordance with. Therefore, the study of cell biomechanics can bring great value to the breakthrough and application of basic sciences. Cell adhesion is one of the important mechanical properties of cells, which refers to the mutual adhesion and interaction between cells, cells and other materials, surfaces, substrates or matrices. For example, the mechanical and chemical coupling between new cells and old cells; the s...

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Problems solved by technology

This method is complicated to make cell probes, and the cells need to be fixed with the probes, which may affect the mechanical properties of the cells themselves, and there are many interference factors. Measuring the adhesion force through the deformation of the probe cantilever beam requires extremely high sensitivity of the equipment, and the measurement accuracy is difficult. ensure

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