Immobilizing device for producing immobilized enzyme or cells and immobilizing method thereof

A technology for immobilizing enzymes and immobilizing carriers, which is applied in the directions of biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc. Guaranteed biological activity, time shortening effect

Pending Publication Date: 2017-10-10
山东绿健生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In most of the preparation methods of immobilized enzymes reported in current patents, a syringe is used to squeeze and drop the mixture of immobilized carrier materials and enzymes into a cross-linking agent for cross-linking and solidification to prepare immobilized enzymes. Methods and tools cannot meet the requirements of industrial production applications

Method used

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  • Immobilizing device for producing immobilized enzyme or cells and immobilizing method thereof
  • Immobilizing device for producing immobilized enzyme or cells and immobilizing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] As introduced in the background technology, there are few studies on the preparation equipment and industrial application of immobilized enzymes in the prior art, which cannot meet the requirements of the industrial production and application of immobilized enzymes or cells. In order to solve the above technical problems, the present invention proposes An immobilization device for producing immobilized enzymes or cells is disclosed, which comprises from top to bottom: a mixer 1 for mixing and dissolving materials, a material distributor 2, a titrator 3 and a curing box 4; Solution preparation, immobilized carrier and microbial cell (or enzyme) mixed solution preparation, immobilized enzyme (or cell) titration and curing functions are integrated, and immobilized enzymes are produced in batches. See details figure 1 .

[0075] Wherein, the material distributor 2 is a three-way diverter valve, and the three-way diverter valve includes an inlet port, an outlet port and a r...

Embodiment 2

[0085] A method for the industrial production of immobilized sucrose isomerase utilizing the immobilized device in Example 1, comprising the steps of:

[0086] (1) Prepare 6% calcium chloride sucrose solution (cross-linking curing agent solution): close the outlet control valve 21 and the reversing port control valve 22 of the material distributor 2, and connect the reversing port control valve with one end of a silicone tube 22, the other end is connected in the curing box 4, close the water outlet 407 of the curing box 4; open the shielding cover 14 of the mixer 1, add 6% (W / V) calcium chloride, 30 % (W / V) white granulated sugar, then open the water inlet valve 15 and add 50L of water; finally, adjust the position of the agitator 12, cover the cover plate 14, turn on the motor 13 to stir and dissolve the crosslinking curing agent solution; the material dissolves After completion, turn off the motor 13, open the reversing port control valve 22 of the material distributor 2, a...

Embodiment 3

[0093] A method for the industrial production of immobilized sucrose isomerase utilizing an immobilized device, comprising the steps of:

[0094] (1) Prepare 3% calcium chloride sucrose solution (cross-linking curing agent solution): close the material distributor 2 outlet control valve 21 and the reversing port control valve 22, and connect the reversing port control valve 22 with one end of a silicone tube , the other end is connected in the curing box 4, close the curing box 4 water outlet 407; open the cover 14 of the mixer 1, add 3% (W / V) calcium chloride, 25% calcium chloride, 25% calcium chloride in the container 11 of the mixer 1 (W / V) white granulated sugar, then open the water inlet valve 15 and add 50L of water; finally, adjust the position of the agitator 12, cover the cover plate 14, turn on the motor 13 to stir and dissolve the crosslinking curing agent solution; the material dissolves completely Finally, turn off the motor 13, open the reversing port control val...

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Abstract

The invention discloses an immobilization device and an immobilization method for producing immobilized enzymes or cells. The device is composed of four main parts: a mixer, a material distributor, a titrator and a curing box. , Immobilized carrier and microbial cell (or enzyme) mixed solution preparation, immobilized enzyme titration and curing functions in one, mass production of immobilized enzymes.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to an immobilization device for producing immobilized enzymes or cells and an immobilization method thereof. Background technique [0002] The industrial application of enzymes is often limited by its high cost and rapid inactivation factors and cannot be applied and promoted as it should be. In order to improve the economic value of enzymes in industrial applications, enzymes are generally immobilized on substrates to make immobilized enzyme. [0003] Immobilized enzyme is a new technology that binds or confines the active enzyme to a certain area with certain materials, but can still carry out the catalytic reaction unique to the enzyme, and can be recycled and reused. Compared with water-soluble enzymes, immobilized enzymes not only maintain their specificity, high efficiency, mildness, and adjustable control of enzyme activity, but also have the advantages of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/02C12N11/10C12N11/04
CPCC12M21/14C12M27/02C12N11/04C12N11/10
Inventor 信成夫罗祖会景文利于丽任尚美刘建
Owner 山东绿健生物技术有限公司
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