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Chinese herbal composition with antitumor activity, preparation method and application thereof

A technology of anti-tumor activity and composition, which is applied in the direction of anti-tumor drugs, drug combinations, and medical preparations containing active ingredients, etc., which can solve the problems of large adverse reactions, high toxicity of chemotherapy drugs, and low survival rate of patients, and achieve The effect of strengthening the immune function of Peiben, inhibiting the growth of tumor cells, and scientific and reasonable proportioning

Inactive Publication Date: 2017-10-10
青海九康中药饮片有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, the current drugs for treating tumors mainly include chemical drugs. However, due to the high toxicity of chemotherapy drugs, they can not selectively kill white blood cells, lymphocytes and other human immune cells while killing tumor cells. As a result, patients often suffer from autoimmune diseases. Complicated infection due to low ability, severe adverse reactions, low survival rate and high cost

Method used

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  • Chinese herbal composition with antitumor activity, preparation method and application thereof
  • Chinese herbal composition with antitumor activity, preparation method and application thereof

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preparation example Construction

[0037] 2. the preparation method of the Chinese medicine compound composition with antitumor activity of the present invention, comprises the following steps:

[0038] The preparation method of the Chinese medicine compound composition with antitumor activity of the present invention comprises the following steps:

[0039](1) Take 60 parts of Codonopsis pilosula, 60 parts of Radix Pseudostellariae, 60 parts of Radix Scrophulariae, 60 parts of Radix Astragali, 30 parts of Seed of Predict, 20 parts of Semen Xanthium, 30 parts of Honeycomb, 20 parts of Shegan, 20 parts of Puffball, 30 parts of geranium seeds, 30 parts of Aesculus chinensis, 30 parts of Baiying, 20 parts of vine pear root, 20 parts of verbena, 20 parts of Viola chinensis, 20 parts of mallow seeds, 10-20 parts of Iwami wear, fish 20 parts of Houttuynia cordata, 60 parts of Glycyrrhizae radix, 20 parts of Caterpillar, add 12 times the amount of ethanol to extract 3 times, each time for 2 hours, combine the extracts,...

Embodiment 4

[0055] Embodiment 4 in vitro anticancer experiment

[0056] 1. In vitro anti-tumor experiment

[0057] Human lung cancer (MCF-7), breast cancer (A-5408), human liver cancer cell SMMC-7721, human gastric cancer (SGC-7901), human leukemia cell HL-60, human intestinal mucinous adenocarcinoma and prostate cancer Strain, adopt thiazolium blue reduction method (MTT) to carry out in vitro antitumor experiment, the extract of the Chinese medicine composition that the embodiment of the present invention 1, embodiment 2, embodiment 3 prepares is test drug, is positive control group with cisplatin , the solvent of the sample, i.e. normal saline, was used as the negative control group. The specific experimental results are shown in Table 1:

[0058] Table 1 The inhibitory effect of Chinese medicine composition on tumor cells (IC 50 is the half inhibitory rate)

[0059]

[0060]

[0061] The results of in vitro anti-tumor experiments in Table 1 show that the traditional Chinese m...

Embodiment 1

[0066] Example 1, the Chinese medicine compound extract prepared in Example 2 and Example 3; sheep red blood cells (provided by Guangzhou Ruite Biotechnology Co., Ltd.); guinea pig serum (provided by Guangzhou Ruite Biotechnology Co., Ltd.); the kit was built in Nanjing Provided by the Institute of Bioengineering; other reagents were of domestic analytical grade.

[0067] 1.3 Experimental Instruments

[0068] Freeze dryer (LABCONCO, USA), UV spectrophotometer (Beijing Labtech Instrument Co., Ltd.), microplate reader (PerkinElmer Co., Ltd.), automatic blood cell analyzer (Sysmex Corporation, Japan), electronic balance (Sartorius Scientific Instruments Ltd.).

[0069] 2. Experimental method

[0070] 2.1 Modeling and administration of immunocompromised mice

[0071] 70 mice, half male and half male, were weighed and numbered, randomly divided into 5 groups, including a blank group, a model group, and the Chinese medicine compound extracts prepared in Example 1, Example 2 and E...

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Abstract

The invention discloses a Chinese herbal composition with antitumor activity, a preparation method and an application thereof. The Chinese herbal composition is prepared from codonopsis pilosula, radix pseudostellariae, radix scrophulariae, astragalus membranaceus, akebia fruit, cocklebur fruit, apiary, blackberry lily, puffball, radix semiaquilegiae, paris polyphylla, solanum lyratum, Chinese actinidia root, vervain, herba violae, chingma abutilon seed, salvia chinensis, houttuynia cordata, liquorice, Chinese starjasmine stem, leech, ground beeltle, gecko and cantharides. An antitumor experiment result proves that the Chinese herbal composition provided by the invention is scientific and reasonable in formula; the experiment result proves that the Chinese herbal composition has strong killing effect for various malignant cells; and the Chinese herbal composition can enhance immunity, has low untoward effect and high safety and is wide in application scope.

Description

technical field [0001] The invention relates to a traditional Chinese medicine, in particular to a compound traditional Chinese medicine composition with anti-tumor activity and its preparation method and application. Background technique [0002] Tumor has become the number one killer threatening human existence in the 21st century. China is one of the countries with high incidence of cancer in the world, and it shows a rapid growth trend every year. Clinically, the current drugs for treating tumors mainly include chemical drugs. However, due to the high toxicity of chemotherapy drugs, they can not selectively kill white blood cells, lymphocytes and other human immune cells while killing tumor cells. As a result, patients often suffer from autoimmune diseases. Complicated infection due to low ability, severe adverse reaction, low survival rate and high cost. [0003] Traditional Chinese medicine has unique advantages in the treatment of tumors. Natural drugs represented by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/88A61K36/896A61P35/00A61K35/583A61K35/62A61K35/64
CPCA61K36/896A61K35/583A61K35/62A61K35/63A61K36/07A61K36/12A61K36/185A61K36/24A61K36/28A61K36/344A61K36/36A61K36/481A61K36/484A61K36/537A61K36/71A61K36/78A61K36/808A61K36/81A61K36/85A61K36/86A61K36/88A61K2236/33A61K2236/39A61K2300/00
Inventor 李成龙李志成程海波孙东东沈卫星李柳曹岗苗筠杰谭佳妮徐长亮
Owner 青海九康中药饮片有限公司
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