Accurate confirming method for antigen immunizing dose for goat anti-human serum preparation

A technology of antigen immunization and determination method, which is applied in the field of bioengineering, can solve the problems of high price of immune antigen and low titer of antiserum, and achieve the effect of reducing antigen immunization dosage, high titer and saving production cost

Active Publication Date: 2017-09-29
海奥斯生物科技镇江有限公司
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Problems solved by technology

[0005] The immunizing antigens used in the current method are expensive and the titer of antiserum is low

Method used

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  • Accurate confirming method for antigen immunizing dose for goat anti-human serum preparation
  • Accurate confirming method for antigen immunizing dose for goat anti-human serum preparation
  • Accurate confirming method for antigen immunizing dose for goat anti-human serum preparation

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Embodiment Construction

[0025] Below in conjunction with example the present invention is described in further detail.

[0026] Antigen dose for the first immunization:

[0027] Benchmark amount: Calculated by 8 parts per billion of sheep weight, such as 50kg sheep, the benchmark amount is 0.4mg.

[0028] The basic amount corresponds to: protein antigen molecular weight 50KD.

[0029] When the molecular weight of the antigen protein is less than 50KD, or greater than 50KD, increase the adjustment coefficient to adjust the dose of antigen immunization.

[0030] When the molecular weight of the antigenic protein is less than 50KD, increase the antigenic amount of the adjustment coefficient, up to 1mg. Wherein, the increment adjustment coefficient=(50-antigen molecular weight KD) / 100. For example, if the molecular weight of an antigen is 20KD and the weight of a sheep is 50kg, then the antigen dose for the first immunization is: reference amount + reference amount * (50-20) / 100.

[0031] When the mo...

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Abstract

The invention discloses an accurate confirming method for an antigen immunizing dose for goat anti-human serum preparation. The method comprises the following steps: (1) confirming the antigen immunizing dose for the first time: datum quantity: calculating eight billionths of the weight of goat; corresponding the basic amount: antigen protein molecular weight 50KD and increasing the accommodation coefficient for adjusting the antigen immunizing dose when the antigen protein molecular weight is less than or more than 50KD; (2) keeping the immunizing dose at the second time being 2 times of the immunizing dose at the first time; (3) keeping the immunizing dose at the third time being 2 times of the immunizing dose at the second time; and (4) keeping the immunizing dose from the fourths time to the last time being consistent to the immunizing dose at the third time. The method has the advantages that the antigen immunizing dose which is unclearly expressed in the original textbook is clearly expressed; the accurate operation is conveniently performed; a basis is established for the industrial production for biotechnological animal antiserum preparation; the antigen is expensive, the antigen immunizing dose can be extremely reduced, and the production cost can be saved; and the immunization is accurate, and the antiserum titer is higher.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for accurately determining the immune dose of an antigen prepared by goat anti-human serum. Background technique [0002] Antigens and antibodies are two important factors in immunological testing, and are also the basic conditions for the entire immune response. Antigen purification is a prerequisite for the preparation of specific antibodies, and the prepared antibodies can be used to purify and detect antigens. There are two types of antibodies, monoclonal and polyclonal. [0003] Antiserum is serum containing a certain type of antibody molecule with specific immune function, generally animal serum prepared after animals are artificially injected with a certain type of antigen. High titer antisera are used in research work as well as in the diagnosis and treatment of diseases. Whether an antigen can cause antibody production depends on the one hand whether...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/06
CPCC07K16/06C07K2317/20
Inventor 袁金城江晓
Owner 海奥斯生物科技镇江有限公司
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