Liaofu variety big fruit sea-buckthorn shoot tip tissue medium formula

A technology of tissue culture medium and seabuckthorn, which is applied in the field of plant tissue culture, can solve the problems of no industrial production reports and formulas, and achieve the effect of convenient and rapid propagation

Inactive Publication Date: 2017-09-15
新疆阿勒泰地区园林场
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Problems solved by technology

In recent years, with the continuous development of seabuckthorn products, it has also greatly promoted people's enthusiasm for building seabuckthorn protection forests. Seabuckthorn has already become a symbolic plant for sand prevention and improvement of soil in my country, and is more and more popular among people. I like it. Along with the ecological development, Xinjiang's forestry and fruit industry is also developing rapidly. The mountain greening and ecological agriculture construction in the Altay area of ​​northern Xinjiang urgently needs a large number of seabuckthorn seedlings. Because it is not limited by seasons and regions, it can not only achieve the purpose of rapid breeding, but also maintain the advantages of good breed characteristics, especially for qualitative breeding of dioecious seabuckthorn, which has great development prospects
[0003] Seabuckthorn tissue culture research has been reported in the literature, but there is no report and formula that can be used for industrial production

Method used

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  • Liaofu variety big fruit sea-buckthorn shoot tip tissue medium formula
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  • Liaofu variety big fruit sea-buckthorn shoot tip tissue medium formula

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Embodiment Construction

[0014] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0015] A kind of Liaofu cultivar Seabuckthorn shoot tip tissue culture medium formula, including three kinds of primary culture medium, callus induction medium and subculture medium, wherein,

[0016] Formula of primary medium: 1 / 2MS, 6-BA0.5mg / L, sucrose 30g / L, agar 6g / L; when preparing, first measure MS medium, add 6-BA, add sucrose, after the sucrose is dissolved, Adjust the pH to 5.6-6.0, add agar, heat to melt the agar, sterilize under high temperature and high pressure (121° C., 25 minutes), and cool to a solid for later use.

[0017] Callus induction medium formula: 1 / 2MS, 6-BA0.5mg / L, IBA0.5mg / L, sucrose 30g / L, agar 6g / L; when preparing, first measure MS medium, add 6-BA , IBA, add sucrose, after the sucrose is dissolved, adjust the pH to 5.6-6.0, add agar, heat to melt the agar, sterilize under high temperature and high pressure (121°C, 2...

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Abstract

The invention discloses a culture medium formula for the shoot tip tissue of the Liaofu variety Hippophae rhamnoides L., which includes three kinds of primary medium, callus induction medium and subculture medium; the medium formula of the present invention is specially suitable for culturing The rapid propagation of the shoot tip tissue of Hippophae rhamnoides L., the first-generation medium promotes tissue growth and lateral bud induction, the callus induction medium can induce the tissue to quickly form callus, and the subculture medium is used for rapid propagation; The synergistic effect of the three medium formulas facilitates the rapid propagation of the shoot tip tissue of the large-fruited Seabuckthorn of the Liaofu variety, laying a certain foundation for future industrial production.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a formula for a culture medium for shoot tip tissue of the Liaofu variety Hippophae rhamnoides large fruit. Background technique [0002] Seabuckthorn (Latin scientific name: Hippophae rhamnoides L.), a plant of the genus Hippophae L. in the family Elaeagnaceae, is a deciduous shrub or small tree with developed root system, dense branches and leaves, strong adaptability, cold resistance, wind and sand resistance. It is a pioneer tree species for soil and water conservation in barren and desertified areas. Seabuckthorn mainly grows in arid and semi-arid areas. It has strong stress resistance and wide adaptability. It is distributed in Northwest, North China, Northeast and Southwest provinces of China. It is a kind of food that combines social, ecological and economic benefits. Precious tree species. In recent years, with the continuous development of seabuckthorn products, it...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 赵英徐航郑兴国韩晓燕刘伟张志刚
Owner 新疆阿勒泰地区园林场
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