Method for screening anaerobion traditional Chinese medicine inhibitor based on Fe-Ta-Cu-B nanoprobe

A nano-probe, anaerobic bacteria technology, applied in measurement devices, instruments, analysis by nuclear magnetic resonance, etc., can solve problems such as the decrease of nuclear magnetic resonance relaxation time

Inactive Publication Date: 2017-09-08
NANCHANG UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As mentioned above, a small amount of Fe-Ta-Cu-B nanomaterials will cause a significant decrease in the NMR relaxation time of water

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] To screen traditional Chinese medicine formulas that have inhibitory or therapeutic effects on acne bacilli (anaerobic bacteria).

[0020] 1) Nano-Fe-Ta-Cu-B immunoprobe preparation:

[0021] Fe-Ta-Cu-B nanoparticles can be purchased from the market, or can be synthesized by physical or chemical methods with reference to literature.

[0022] Silica-coated nano-Fe-Ta-Cu-B: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-Fe-Ta-Cu-B and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85˚C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated thre...

example 2

[0027] To screen traditional Chinese medicine formulas that have inhibitory or therapeutic effects on Clostridium difficile (anaerobic bacteria).

[0028] 1) Nano-Fe-Ta-Cu-B immunoprobe preparation:

[0029] Fe-Ta-Cu-B nanoparticles can be purchased from the market, and can also be synthesized by physical and chemical methods with reference to literature.

[0030] Silica-coated nano-Fe-Ta-Cu-B: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-Fe-Ta-Cu-B and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85˚C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention provides a method for screening an anaerobion traditional Chinese medicine inhibitor based on a Fe-Ta-Cu-B nanoprobe, and belongs to the technical field of development and application of traditional Chinese medicine. The method is characterized in that the Fe-Ta-Cu-B nanoprobenanoprobe is prepared and used as the basis for screening an anaerobion traditional Chinese medicine formula; the property that a paramagnetic Fe-Ta-Cu-B nanoprobenanoprobe prepared by antibody-coating is capable of performing specific binding with target bacteria and the influence of the paramagnetic property of Fe-Ta-Cu-B on nuclear magnetic resonance relaxation time are utilized for detecting whether a sample contains the target bacteria. The paramagnetic Fe-Ta-Cu-B nanoprobe shows a linear relation with the nuclear magnetic resonance relaxation time within a certain range, that is, if the nano Fe-Ta-Cu-B content is high, the nuclear magnetic resonance spin-lattice relaxation time and spin-spin relaxation time of the sample are small, and the target bacteria can be quantitatively detected within a certain range, and as a result, the bacteria inhibition effect of the traditional Chinese medicine formula can be indirectly assessed. The method is applicable to the screening of the traditional Chinse medicine formula capable of inhibiting anaerobion; and the development of a traditional Chinese medicine product is sped up.

Description

technical field [0001] The invention relates to a screening method of traditional Chinese medicine formula. Background technique [0002] Antibiotics can also cause damage to the human body while sterilizing bacteria, affecting liver, kidney function, and gastrointestinal tract reactions. Abuse of antibiotics may lead to the occurrence of drug resistance in some bacteria, and the treatment of infections will become very difficult. At present, there is almost no antimicrobial drug without drug resistance. It is of positive significance to develop traditional Chinese medicines and formulas with antibacterial effects. [0003] Many traditional Chinese medicines and prescription medicines have antibacterial and anti-inflammatory effects, and the active ingredients and molecular structures of some Chinese herbal medicines have also been fully or partially studied. For example, berberine (berberine), the main component of Coptis chinensis and Phellodendron phellodendron for dys...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08
CPCG01N24/088
Inventor 张锦胜万益琴彭红巫小丹邹灯超郑洪立赵茹
Owner NANCHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap