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Application of grape earliness gene VvWRKY13 in regulation and control of biosynthesis of ethylene in plants

A biosynthetic and genetic technology, applied in angiosperms/flowering plants, applications, plant products, etc., can solve problems such as limited attention, and achieve the effect of promoting early fruit ripening, shortening the expansion period of young fruit, and shortening the fruit development period

Active Publication Date: 2017-09-08
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, research on fruit growth and development mainly focuses on the second growth cycle, that is, the stage from fruit color change to ripening, while the attention to the first two periods is limited.

Method used

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  • Application of grape earliness gene VvWRKY13 in regulation and control of biosynthesis of ethylene in plants
  • Application of grape earliness gene VvWRKY13 in regulation and control of biosynthesis of ethylene in plants
  • Application of grape earliness gene VvWRKY13 in regulation and control of biosynthesis of ethylene in plants

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Experimental program
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Effect test

Embodiment 1

[0050] clarified by the present invention VvWRKY13 Proof of the function and application of the gene in ethylene biosynthesis, specifically including the following steps:

[0051] 1, VvWRKY13 Transformation and selection of overexpressed tomato:

[0052] (1) A certain amount of commercially available ornamental tomato cultivar ‘Micro-Tom’ was soaked and disinfected with 75% ethanol for 30 s, and quickly discarded. Soak and disinfect with 4% sodium hypochlorite for 15 minutes, wash with sterile water 7-8 times after disinfection, fully wash each time to completely remove residual disinfectant. After the seeds are washed, the seeds are sown on the seed germination medium (1 / 2MS). Cultivate in the dark for 3-4 days until the seeds germinate, then move to 25 °C after germination, and cultivate under the conditions of a photoperiod of 16 h / 8 h (light / dark). After the cotyledons are grown, the seedlings just before the cotyledons are opened and the true leaves are not grown are ...

Embodiment 2

[0099] clarified by the present invention VvWRKY13 The proof of function and application in ethylene biosynthesis includes the following steps:

[0100] 1, VvWRKY13 Transformation and selection of overexpressed tomato:

[0101] (1) A certain amount of seeds of the commercially available cultivar 'Ailsa Craig (AC)' was taken, soaked in 75% ethanol for 30 s, and discarded quickly. Soak and disinfect with 4% sodium hypochlorite for 15 minutes, wash with sterile water 7-8 times after disinfection, fully wash each time to completely remove residual disinfectant. After the seeds are washed, the seeds are sown on the seed germination medium (1 / 2MS). Cultivate in the dark for 3-4 days until the seeds germinate, then move to 25 °C after germination, and cultivate under the conditions of a photoperiod of 16 h / 8 h (light / dark). After the cotyledons are grown, the seedlings just before the cotyledons are opened and the true leaves are not grown are used for transformation.

[0102] (...

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Abstract

The invention provides application of a grape earliness gene VvWRKY13 in regulation and control of biosynthesis of ethylene in plants. The application comprises the steps of carrying out leaf dish infestation on an EHA105 agrobacterium tumefaciens strain carrying recombinant plasmids so as to obtain an over-expressed tomato material of VvWRKY13, and carrying out repeated selfing so as to obtain at least three independent pure transgenic lines; and detecting the ethylene generation amounts of transgenic tomato lines in different growth and development periods, the expression amount of key enzyme genes for synthesis of ethylene, relevant phenotypic characters of ethylene, lengths of transgenic fruits in different growth cycles and relevant quality indexes of mature fruits. An experiment result proves that by applying VvWRKY13 to the regulation and control of the key enzyme genes of ethylene and the biosynthesis of ethylene, the expansion period of young fruits of transgenic tomatoes is shortened, the early maturing of the fruits is promoted, the quality functions of the mature fruits are not changed, and the theoretical basis and the experimental foundation are provided for the early-maturing breeding of berry fruits such as the tomatoes.

Description

technical field [0001] The invention belongs to the technical field of mining and functional identification of grape early-maturing favorable genes, in particular to grape early-maturing genes VvWRKY13 Applications in the regulation of ethylene biosynthesis in plants. Background technique [0002] Grape, as a kind of universal fruit in the world, is popular all over the world with its unique winemaking characteristics. With economic growth, China's wine consumption is on the rise, and the area under grape cultivation has increased accordingly. According to the statistics released by the International Organization of Vine and Wine (OIV), in 2014 China’s grape planting area for wine making was 799,000 hectares, accounting for 11% of the global grape planting area. With 792,000 hectares, France ranks second in the world. At present, the grape industry has become an important industry in my country's agricultural industrialization. Therefore, discovering the molecular regula...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00
CPCC12N15/8249
Inventor 马倩刘新侯丽霞张岁芳
Owner QINGDAO AGRI UNIV
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