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Multiplex test piece device and its operation method

An operation method and test piece technology, which are applied in chemical instruments and methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve complex and time-consuming problems, and achieve the effect of saving time and simplifying experimental operations.

Active Publication Date: 2019-11-05
CRACKERBIO INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as the volume of the reaction vessel becomes smaller and the distance between the wells is closer, it is complicated and time-consuming to design a special mechanical mechanism of the dispenser or path to deliver to each reaction vessel individually

Method used

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  • Multiplex test piece device and its operation method
  • Multiplex test piece device and its operation method
  • Multiplex test piece device and its operation method

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Embodiment Construction

[0059] The invention provides a multiplex test piece device and its operating method, which can be widely used in different types of reaction tests. In the following, the terms used in the specification will be defined first.

[0060] "Reagent" may refer to a formulation or preparation of several components for a particular test / test. For example, in assays using polymerase chain reaction, detection reagents include a pair of primers, enzymes, dNTPs, fluorescent reporters, and salts. In the application, different primer pairs and fluorescent reporters may be added to the reaction vessel first, and then the sample is mixed with enzymes, dNTPs and other additives before being added to the reaction vessel.

[0061] "Sample" refers to a nucleic acid sample to be tested. For example, a sample can be nucleic acid fragments (including DNA or RNA, etc.) extracted from blood, tissue, saliva, and other sources.

[0062] "Analysis" or "test" may refer to one or more experiments or tes...

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Abstract

A multiplex slide plate device and an operation method thereof are provided. The multiplex slide plate device includes a slide plate and a sacrificial layer. The slide plate has reaction vessels arranged in an array, wherein each of the reaction vessels has an opening portion and a bottom portion. The sacrificial layer has a microfluidic channel, wherein the microfluidic channel has an injection channel, a main channel and a distal channel connected to each other. The sacrificial layer is assembled to the slide plate, wherein the main channel faces the opening portion. A sample solution is injected into the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into each of the reaction vessels while flowing through the main channel. The sample can be loaded into each reaction vessel of the slide plate quickly and uniformly through the multiplex slide plate device and the operation method of the invention, and all of the reaction vessels can be filled in an extremely short time by single pipetting.

Description

technical field [0001] The present invention relates to a multiplex test piece device and its operation method, to be applied to molecular biology detection, especially to a kind of multiplex test piece device used for polymerase chain reaction (PCR), which can be pre-filled with polymerase chain reaction reagents and its method of operation. Background technique [0002] In the field of molecular biological detection, it is often necessary to test multiple items for one sample. For example, the genotypes of several single nucleotide polymorphisms (SNPs) in a sample are tested by polymerase chain reaction detection, or the expression levels of multiple genes in a sample are tested. In this case, several DNA or RNA assays will be required to form a panel. In the PCR test, at least two specific DNA primer molecules are included in each set of test reagents (in some PCR tests, target-specific reporter probes (reporter probes) are additionally included), and this pair of prime...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCB01L3/5027B01L7/52B01L2200/10
Inventor 邱创汎味正唯张钰许铭权
Owner CRACKERBIO INC
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