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Application of shikonin in preparing drug for treating lung cancer

A shikonin and drug technology, applied in the field of anti-tumor drug preparations, can solve the problems of cancer cell aging and anti-tumor effects, and achieve the effect of inducing cancer cell aging

Inactive Publication Date: 2017-09-08
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report that shikonin can induce cancer cell senescence and play an anti-tumor effect.

Method used

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  • Application of shikonin in preparing drug for treating lung cancer
  • Application of shikonin in preparing drug for treating lung cancer
  • Application of shikonin in preparing drug for treating lung cancer

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0022] Experimental example 1: Inhibitory effect of shikonin on the viability of lung cancer cells

[0023] When the cell growth state is good, inoculate 2000 cells / well in a 96-well plate and culture overnight. When the cell adhesion state is good, discard the original medium, and then follow the designed drug concentration: shikonin concentration 0 , 1, 2, 4, 6, 8μM for medium change administration, act on A549 cells for 72h, stop the culture, suck out the medium, add 100μl of 0.5mg / ml MTT solution, incubate at 37℃ for 4-6 hours, discard the MTT solution , and finally add 150 μl of DMSO to shake and mix well, place in a microplate reader, and detect the absorbance value at a measuring wavelength of 490 nm. The result is as figure 1 As shown, shikonin can significantly inhibit the growth of lung cancer cell A549.

experiment example 2

[0024] Experimental example 2: Shikonin up-regulates the expression level of p53 protein in lung cancer cells

[0025] A549 cells in the logarithmic growth phase were inoculated into 6-well culture plates at 6 × 104 cells / well, and cultured overnight. 1, 2 μM) of fresh culture medium for 72 hours, digested, collected cells, centrifuged, washed twice with PBS, washed off the supernatant, then added appropriate amount of RIPA and 1% protease inhibitor PMSF, lysed cells for 45 minutes, collected the supernatant The supernatant was used to determine the protein content, and the expression of p53 protein was detected by Western blotting. The result is as figure 2 As shown, shikonin can significantly up-regulate the expression level of p53 protein in A549 cells after acting on it.

experiment example 3

[0026] Experimental Example 3: Shikonin Induces Senescence of Lung Cancer Cells

[0027] A549 cells in the logarithmic growth phase were inoculated in 6-well plates at 6×104 cells / well, and cultured overnight. After the medium was cultured for 72 hours, the old medium was discarded, washed twice with PBS, fixed with 1X Fixative solution for 10 minutes, discarded, then washed twice with PBS, and then stained with β-galactosidase solution, incubate overnight, and detect the degree of cell senescence under a fluorescent inverted microscope. The more blue cells, the deeper the blue of the cells, and the more serious the degree of cell senescence. The result is as image 3 As shown, after the effect of shikonin, the degree of cell senescence increased in a dose-dependent manner.

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Abstract

The invention relates to application of shikonin in preparing drug for treating lung cancer. The chemical structural formula of shikonin is shown as a following formula (I). Shikonin can effectively activate P53 pathways in cancer cells, induce senescence of the cancer cells and play a role in treating the lung cancer.

Description

[0001] Technical field: [0002] The invention relates to a pharmaceutical preparation containing organic active ingredients, in particular to an antitumor drug preparation of a ketone compound from a plant of the genus Comfrey. [0003] Background technique: [0004] Tumors, especially malignant tumors, are one of the major diseases that seriously threaten human life and health. Its lethality to humans is second only to cardiovascular and cerebrovascular diseases, ranking second in the world. It is still a major disease problem that countries all over the world are still facing. According to statistics, the incidence of cancer in my country has increased by 69% in less than 20 years, and the death rate has increased by 29.4%. health problems. Therefore, finding effective cancer treatment drugs and further improving the clinical treatment of tumor systems have become urgent research issues. As a big country of Chinese herbal medicine, my country has a unique advantage in extra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00
CPCA61K31/122
Inventor 刘中秋卢琳琳郑宏明姬利延冯潜王莹戚笑笑吴鹏朱丽君
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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