Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for fermenting alcohol by mixing corn mash and soybean aqueous enzymatic hydrolysate

A technology for mixing fermentation and hydrolyzate, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of complex production process and high cost, improve alcohol yield, solve high cost, and solve environmental pollution. effect of the problem

Inactive Publication Date: 2017-09-05
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the rapid development of my country's economy, the contradictions among energy, environment and agriculture have become increasingly prominent, and the demand for alcohol is also increasing. At present, the production of alcohol by fermentation mainly uses grains, potatoes and honey as raw materials, and the production process is complex and high cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for fermenting alcohol by mixing corn mash and soybean aqueous enzymatic hydrolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) The preparation process of corn saccharification used in this experiment is as follows: corn flour and water are mixed at a ratio of 1:4 to make a mixture, and then stand for 30 minutes after mixing. Add amylase (10u / g starch) based on 70% starch content of corn flour, raise the temperature to 90°C, and keep it warm for 30min. That is saccharification;

[0021] (2) The water enzymatic hydrolyzate extraction process used in this experiment is: mix puffed soybean powder and deionized water at a ratio of 1:5, add Alcalase alkaline protease, enzymatically hydrolyze at 55°C for 3 hours, then inactivate the enzyme and centrifuge to remove soybean oil , emulsion and the insoluble residue in the lower layer to obtain the hydrolyzate;

[0022] (3) Trichoderma pseudokonii was selected as the fermentation strain, and under sterile conditions, it was amplified and cultivated step by step on a test tube slope, a shaker flask, a first-level seed tank, and a second-level seed tan...

Embodiment 2

[0025] (1) The preparation process of corn saccharification used in this experiment is as follows: corn flour and water are mixed at a ratio of 1:4 to make a mixture, and then stand for 30 minutes after mixing. Add amylase (10u / g starch) based on 70% starch content of corn flour, raise the temperature to 90°C, and keep it warm for 30min. That is saccharification;

[0026] (2) The water enzymatic hydrolyzate extraction process used in this experiment is: mix puffed soybean powder and deionized water at a ratio of 1:5, add Alcalase alkaline protease, enzymatically hydrolyze at 55°C for 3 hours, then inactivate the enzyme and centrifuge to remove soybean oil , emulsion and the insoluble residue in the lower layer to obtain the hydrolyzate;

[0027] (3) Select the cellulase-producing bacterium Pseudomonas konshii as the fermentation strain. Under sterile conditions, the OD value of the number of bacteria is obtained by step-by-step amplification and cultivation of the test tube i...

Embodiment 3

[0030] (1) The preparation process of corn saccharification used in this experiment is as follows: corn flour and water are mixed at a ratio of 1:4 to make a mixture, and then stand for 30 minutes after mixing. Add amylase (10u / g starch) based on 70% starch content of corn flour, raise the temperature to 90°C, and keep it warm for 30min. That is saccharification;

[0031] (2) The water enzymatic hydrolyzate extraction process used in this experiment is: mix puffed soybean powder and deionized water at a ratio of 1:5, add Alcalase alkaline protease, enzymatically hydrolyze at 55°C for 3 hours, then inactivate the enzyme and centrifuge to remove soybean oil , emulsion and the insoluble residue in the lower layer to obtain the hydrolyzate;

[0032] (3) Select the cellulase-producing bacterium Pseudomonas konshii as the fermentation strain. Under sterile conditions, the OD value of the number of bacteria is obtained through the step-by-step amplification culture of the test tube ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for fermenting alcohol by mixing corn mash and soybean aqueous enzymatic hydrolysate. The method includes the steps: (1) blending corn powder and water in proportion to prepare mixed liquid, adding amylase, increasing temperature to 90 DEG C, keeping the temperature for 30 minutes, adding an appropriate amount of saccharifying enzyme when the temperature is reduced to 56 DEG C, performing heat preservation and saccharification for 18 hours and saccharifying supernate to obtain saccharifying mash; (2) mixing puffing soybean powder with deionized water, adding Alcalase alkaline protease, performing enzymolysis at the temperature of 55 DEG C for 3 hours, killing enzyme, performing centrifugation and removing soybean oil, emulsion and lower insoluble residues to obtain the hydrolysate; (3) selecting Trichoderma pseudokoningi as a fermentation strain and performing step-by-step scale-up culture under an aseptic condition; (4) adding 10% liquid fermentation strains into the corn saccharifying mash, adding equivalent aqueous enzymatic hydrolysate, fermenting the corn saccharifying mash and the hydrolysate for 72 hours under the condition of the culture temperature of 30 DEG C, the tank pressure of 0.8kg / cm<2>, the stirring speed of 130r / min and the pH (potential of hydrogen) value of 4.5, and producing the alcohol by distillation. The method has the advantages of short fermentation time, low needed cost, high alcohol purity and the like.

Description

technical field [0001] The invention belongs to an ethanol production method, and mainly relates to a method for fermenting ethanol by mixing corn millet and soybean water enzymatic hydrolyzate. Background technique [0002] With the rapid development of my country's economy, the contradictions among energy, environment and agriculture have become increasingly prominent, and the demand for alcohol is also increasing. At present, the production of alcohol by fermentation mainly uses grains, potatoes and honey as raw materials, and the production process is complex and high cost. At the same time, the aqueous enzymatic method is a new oil extraction method for simultaneously extracting oil and protein from oil plants, but the large amount of hydrolyzate produced during the oil extraction process is an important factor restricting the industrialization of enzymatic oil extraction. Therefore, from the two aspects of developing new energy sources and controlling environmental pol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/06C12P19/20C12P19/14C12P21/06C12R1/885
CPCC12P7/065C12P19/14C12P19/20C12P21/06Y02E50/10
Inventor 李杨齐宝坤董济萱江连洲隋晓楠王中江丁俭孙红波
Owner NORTHEAST AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com