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Cultivation method and application of cordyceps militaris liquid fermentation culture

A technology of liquid fermentation and cultivation methods, applied in the application, liquid fertilizer, botanical equipment and methods, etc., can solve the problems such as the inability of liquid bacteria to supplement oxygen regularly, the production efficiency is not high, the bacteria growth time is slow, etc. Reduced contamination rates, improved production efficiency and yield, reduced degradation effects

Inactive Publication Date: 2017-09-01
天津市东方中滨农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of cultivating in this shaker, oxygen cannot be regularly supplemented to the liquid bacteria, and the liquid bacteria can only fill less than 30% of the volume of the shaker flask. Low seed concentration and low production efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] Embodiment (fermentation strain)

[0027] The first step is to prepare the shaker culture medium

[0028] The liquid medium is calculated in parts by weight, including: 9.5 parts of potatoes, 0.9 parts of glucose, 0.45 parts of peptone, 0.45 parts of yeast extract powder, 0.093 parts of potassium dihydrogen phosphate, 0.047 parts of magnesium sulfate, 0.094 parts of defatted silkworm chrysalis powder, and 54 parts of water , Vitamin B10.00095 parts.

[0029] The specific operation is as follows: Weigh 200g of potatoes, boil for 30 minutes, filter out the residue, and obtain potato liquid, add water to the potato liquid to make up to 1137ml, then add 18.9g of glucose, 9.5g of peptone, 9.5g of yeast extract powder, potassium dihydrogen phosphate 2.0g, magnesium sulfate 1.0g, degreased silkworm chrysalis powder 2.0g, vitamin B1 0.02g, dissolve and stir evenly and divide into 500L Erlenmeyer flasks, each bottle is about 280ml, put the liquid Erlenmeyer flasks to sterilize ...

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Abstract

The invention discloses a cultivation method and application of a cordyceps militaris liquid fermentation culture. The method comprises the following steps of (1) inoculating a pure culture of cordyceps militaris into a liquid cultivation solution, and carrying out shake cultivation to obtain a liquid culture at the early stage of germination; (2) trans-inoculating the liquid culture obtained through shaking into fermentation cultivation liquid, and feeding sterile air for carrying out fermental cultivation to obtain the fermentation culture. The invention also provides a high-efficient culture cultivation solution formula. The culture activity is improved, the fungus ball breeding speed is fast, the pollution rate is low, and the cycle of cultivating bacteria is shortened, so that the production efficiency and the yield of cordyceps militaris fruiting bodies are improved.

Description

technical field [0001] The invention relates to the technical field of artificially cultivating fungi by applying the principles of biotechnology, in particular to the cultivation of Cordyceps militaris, and more specifically to a cultivation method and application of a liquid fermentation strain of Cordyceps militaris. Background technique [0002] Cordyceps militaris ( Cordyceps militaris ) belongs to Fungi, Ascomycota, Ascomycetes, Sordariomycetidae, Hypocreales, Clavcipitaceae, Cordyceps ( Cordyceps). Cordyceps militaris is a very well-known ascomycete fungus for both medicine and food, and it is also the main source of cordycepin, which has high development and utilization value. Because wild Cordyceps is very rare and resources are extremely scarce, in order to meet market demand, the artificial cultivation of Cordyceps is being actively promoted, and the active ingredients of artificially cultivated Cordyceps are close to wild, and some of them are even several time...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00A01G1/04
CPCC05B7/00C05G5/23C05C11/00C05D5/00C05F1/005C05F11/00C05F11/08C05F11/10
Inventor 沈中奇李盈
Owner 天津市东方中滨农业科技有限公司
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